| 汪苏杭，王诗琦，吴洪鑫，钟梓春，何柳燕，郭玉静，金丰良，许小霞，庞锐,2025,三种稻飞虱热激蛋白的全基因组鉴定与表达特征分析[J].环境昆虫学报,(5):1374-1388 |
| 三种稻飞虱热激蛋白的全基因组鉴定与表达特征分析 |
| Genome-wide identification and expression characteristics of heat shock proteins in three rice planthopper species |
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| DOI： |
| 中文关键词: 稻飞虱 热激蛋白 基因家族 表达特征 |
| 英文关键词:Rice planthopper heat shock protein gene family expression characteristics |
| 基金项目:国家自然科学基金（32172498，31972345） |
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| 中文摘要: |
| 热激蛋白（Heat shock protein）是不可或缺的分子伴侣蛋白，在昆虫受到应激刺激时发挥着重要作用。已有研究表明，热激蛋白在3种稻飞虱的生长发育、应对非生物胁迫、宿主互作、抗药性形成中发挥重要作用。本研究系统鉴定并分析了褐飞虱Nilaparvata lugens、白背飞虱Sogatella furcifera、灰飞虱Laodelphax striatellus中热激蛋白基因家族成员。分别在3种稻飞虱发现了70个、55个、86个热激蛋白，利用大量外群HSP基因构建的系统发育树表明，稻飞虱中热激蛋白可以分为sHSP、CCT、HSP60、HSP10、DNAJ、HSP90、HSP70、HSP100共8个类别，其中DNAJ数量最多，HSP10、HSP60、HSP90、HSP100数量在1~4个；不同家族间分子量、氨基酸数量、编码蛋白序列长度差异较大，同一家族内差异较小。此外共线性分析结果显示3种稻飞虱间的同一家族基因分布具有明显相似性；根据保守基序和结构域的系统分析，DNAJ家族其他功能结构域数量最多，HSP70、HSP90、HSP10、CCT、HSP60家族均高度保守。之后，根据转录组数据分析总结了3种稻飞虱HSP基因不同发育阶段、组织、不同温度胁迫的表达模式，并实验验证了部分HSP基因不同温度胁迫下的表达。本研究为深入理解飞虱类热激蛋白基因家族的功能多样性、进化机制以及其在环境适应中的作用提供了理论基础和基因资源。 |
| 英文摘要: |
| Heat shock proteins (HSPs) are indispensable molecular chaperones that play critical roles in insects under various stress conditions. Previous studies have demonstrated that HSPs are involved in the growth, development, responses to abiotic stress, host interactions, and insecticide resistance in the three major rice planthoppers. In this study, we systematically identified and characterized the HSP gene family members in the brown planthopper (Nilaparvata lugens), the white-backed planthopper (Sogatella furcifera), and the small brown planthopper (Laodelphax striatellus). A total of 70, 55, and 86 HSP genes were identified in these three species, respectively. Phylogenetic analysis incorporating a large set of outgroup HSP genes revealed that rice planthopper HSPs can be classified into eight families: sHSP, CCT, HSP60, HSP10, DNAJ, HSP90, HSP70, and HSP100, with DNAJ being the most abundant. In contrast, HSP10, HSP60, HSP90, and HSP100 families each contained only 1~4 members. Substantial variation was observed in molecular weight, amino acid number, and protein sequence length among different HSP families, whereas members within the same family were relatively conserved. Collinearity analysis indicated that HSP family members exhibited highly similar chromosomal distributions across the three rice planthoppers. Systematic analysis of conserved motif and domain further showed that DNAJ family members possessed the greatest diversity of additional functional domains, whereas HSP70, HSP90, HSP10, CCT, and HSP60 families were highly conserved. Moreover, transcriptome-based expression profiling revealed distinct expression patterns of HSP genes across different developmental stages, tissues, and under temperature stress in the three planthopper species, which were further validated for selected HSP genes under different thermal conditions. Collectively, this study provides fundamental insights and valuable genetic resources for understanding the functional diversity, evolutionary dynamics, and ecological adaptation roles of the HSP gene families in planthoppers. |
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