《环境昆虫学报》

作者	单位
黄敏君，舒本水，吴仲真，唐睿	1．仲恺农业工程学院农业与生物学院,广州 510225；2．广东省科学院动物研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州 510260

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中文摘要:

昆虫瞬时受体电位（Transient receptor potential，TRP）通道在多感官信号整合中起核心作用，其功能多样性可补偿气味受体（Olfactory receptors，OR）在产卵等复杂行为调控中的局限性。本研究以柑橘木虱Diaphorina citri为对象探究其TRP家族对复杂产卵行为的调控与嗅觉回补，通过化学分析、转录组、进化分析、分子模拟对接手段结合，鉴定寄主九里香中挥发性气味化合物，分析内酯类关键配体与柑橘木虱TRP通道的互作关系。通过气相色谱-质谱联用分析九里香Murraya exotica不同叶龄组织的挥发性成分，发现嫩芽、嫩叶和老叶的挥发性气味组成存在显著差异。其中，邪蒿内酯（Seselin）仅在嫩芽和嫩叶中检出，化合物相关性分析显示异橙皮内酯（Isoauraptene）富集情况与邪蒿内酯相似，可能为另一种潜在的TRP识别活性成分。基于转录组数据，从柑橘木虱中鉴定出12个TRP通道，分属TRPC、TRPV、TRPA、TRPN、TRPP、TRPML和TRPM共7个亚家族，系统发育树显示柑橘木虱TRPs与近缘物种同源基因进化关系保守。表达量分析结果表明TRPP亚家族成员的DcPkd2表达量显著高于其他触角上的基因，结构分析显示DcPkd2属Mplasa_alph_rch superfamily，其结构可能参与嗅觉调控。而TRPA家族中的DcTRPA1、DcPain和DcWtrw都具有高度保守结构。分子对接结果显示，DcPkd2与邪蒿内酯和异橙皮内酯均能形成稳定的结合构象。柑橘木虱可能通过TRP家族的DcPkd2通道特异性识别九里香嫩芽/叶组织挥发物邪蒿内酯和异橙皮内酯，编码内酯类化合物以补偿OR功能，共同调控雌虫在嫩芽上的产卵定位。本研究首次建立柑橘木虱TRP通道-植物挥发物互作模型，为基于嗅觉干扰的害虫防控策略提供新靶标，同时为解析半翅目昆虫产卵定位的嗅觉补偿机制提供了新视角。

英文摘要:

Insect Transient Receptor Potential (TRP) channels play a central role in multisensory signal integration, and their functional diversity can compensate for the limitations of Olfactory Receptors (ORs) in regulating complex behaviors such as oviposition. In this study, Diaphorina citri was used as the research object to explore the regulation of its TRP family on complex oviposition behavior and olfactory compensation. By combining chemical analysis, transcriptomics, evolutionary analysis, and molecular docking simulations, the volatile odor compounds in the host plant Murraya exotica were identified, and the interaction between key lactone ligands and D. citri TRP channels was analyzed. Volatile components in M. exotica tissues of different leaf ages were analyzed using gas chromatography-mass spectrometry (GC-MS). Significant differences were found in the composition of volatile odors among tender buds, young leaves, and old leaves. Among them, seselin was only detected in tender buds and young leaves. Compound correlation analysis showed that the enrichment of isoauraptene was similar to that of seselin, suggesting that it might be another bioactive ligand for TRP recognition. Based on transcriptome data, 12 TRP channels were identified in D. citri, belonging to 7 subfamilies: TRPC, TRPV, TRPA, TRPN, TRPP, TRPML, and TRPM. The phylogenetic tree showed that the evolutionary relationships between D. citri TRPs and homologous genes in related species were conserved. Expression level analysis indicated that DcPkd2, a member of the TRPP subfamily, exhibited significantly higher expression than that of other genes in the antennae. Structural analysis revealed that DcPkd2 belonged to the Mplasa_alph_rch superfamily, and its structure might be involved in olfactory regulation. In the TRPA family, DcTRPA1, DcPain, and DcWtrw all displayed highly conserved structures. Molecular docking results showed that DcPkd2 could form stable binding conformations with both seselin and isoauraptene. D. citri may specifically recognize the volatile compounds seselin and isoauraptene from M. exotica tender buds or leaves through the TRP family member DcPkd2, encoding lactone compounds to compensate for OR functions, and jointly regulating the oviposition positioning of female adults on tender buds. This study established, for the first time, a TRP channel-plant volatile interaction model in D. citri, providing a new target for pest control strategies based on olfactory interference and a new perspective for understanding the olfactory compensation mechanism in oviposition positioning of Hemipteran insects.

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