| 周军辉，罗文芳，许建军，褚 栋，何 伟，杨 坤,2025,短时高温胁迫下番茄潜叶蛾及其体内菌群的耐热性响应机制[J].环境昆虫学报,(4):1123-1134 |
| 短时高温胁迫下番茄潜叶蛾及其体内菌群的耐热性响应机制 |
| Thermotolerance response mechanisms of Tuta absoluta and its microbiota under short-term high-temperature stress |
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| DOI： |
| 中文关键词: 番茄潜叶蛾 高温胁迫 耐热性 Wolbachia 代谢调控 |
| 英文关键词:Tuta absoluta high-temperature stress thermotolerance Wolbachia metabolic regulation |
| 基金项目:青岛市科技惠民示范专项（24-1-8-xdny-10-nsh） |
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| 中文摘要: |
| 全球气候变暖导致的极端高温对入侵昆虫的适应性进化提出了严峻挑战，而大多数入侵昆虫的耐热性响应机制及体内菌群应对高温胁迫变化依然缺乏探究。本研究以重大入侵昆虫番茄潜叶蛾Tuta absoluta为对象，系统解析其耐热性响应机制以及短时高温热激对其整虫体内菌群（包括肠道、生殖腺、口器等），尤其是共生菌Wolbachia的影响。通过梯度热激实验（26℃、36℃、44℃）发现，44℃处理3 h显著降低雌成虫存活率至20.33%，而36℃处理无显著影响；不同温度处理后，番茄潜叶蛾雌成虫寿命无显著性差异；3种抗氧化酶（过氧化氢酶CAT，过氧化物酶POD以及超氧化物歧化酶SOP）活性在高温下未发生显著变化，提示其耐热性不依赖传统抗氧化系统。16S rRNA基因测序表明，高温显著改变菌群结构：变形菌门Proteobacteria占比超83.62%，共生菌Wolbachia在雌成虫高温组丰度显著提升至70.40%（常温组<42.18%），且菌群α多样性（Shannon指数下降64%）与功能发生改变。KEGG分析显示，高温下菌群碳水化合物代谢、能量代谢通路显著激活，并伴随抗生素合成基因富集，暗示其可能通过调控宿主能量稳态与抑制病原菌增强耐热性。本研究揭示了番茄潜叶蛾及其体内菌群应对短时高温热激的响应，为进一步探究Wolbachia等共生细菌对番茄潜叶蛾耐热性影响打下理论基础。 |
| 英文摘要: |
| Global warming-induced extreme high temperatures pose severe challenges to the adaptive evolution of invasive insects, while the thermotolerance response mechanisms of most invasive insects and the changes in their resident microorganism in response to high-temperature stress underexplored. This study systematically investigated the thermotolerance response mechanism of the major invasive pest Tuta absoluta (Tomato Leafminer) and the impact of short-term high-temperature stress on its resident microorganism (including gut, reproductive glands, mouthparts, etc.), particularly on the endosymbiont Wolbachia. Gradient heat shock experiments (26°C, 36°C, 44°C) revealed that 44°C treatment for 3 hours significantly reduced female adult survival rate to 20.33%, while 36°C had no significant effect; female adult lifespan showed no significant differences after different temperature treatments; the activities of three antioxidant enzymes (Catalase CAT, Peroxidase POD, and Superoxide Dismutase SOD) did not change significantly under high temperature, suggesting its thermotolerance does not rely on the traditional antioxidant system. 16S rRNA gene sequencing indicated that high temperature significantly altered the microbial community structure: Proteobacteria accounted for over 83.62%, the abundance of the symbiont Wolbachia in the high-temperature female adult group significantly increased to 70.40% (ambient temperature group < 42.18%), and microbial α-diversity (Shannon index decreased by 64%) and function were altered. KEGG analysis showed that microbiota pathways related to carbohydrate metabolism and energy metabolism were significantly activated under heat stress, accompanied by enrichment of antibiotic biosynthesis genes, implying that Wolbachia may enhance heat tolerance by regulating host energy homeostasis and suppressing pathogens. This study reveals the responses of T. absoluta and its resident microorganism to short-term high-temperature heat shock, laying a theoretical foundation for further investigation into the influence of symbiotic bacteria like Wolbachia on the thermotolerance of T. absoluta. |
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