| 温雪梅，邵雪花，何翔宇，秦子昕，梁 赫，徐玉辉，路 伟,2025,双香豆素抑制SL-221细胞增殖的差异代谢物分析[J].环境昆虫学报,47(3):883-893 |
| 双香豆素抑制SL-221细胞增殖的差异代谢物分析 |
| Differential metabolite analysis of SL-221 cell proliferation inhibited by dicoumarin |
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| DOI： |
| 中文关键词: 双香豆素 斜纹夜蛾 代谢组学 细胞活性 代谢通路 |
| 英文关键词:Dicoumarin Spodoptera litura metabonomics cell viability metabolic pathways |
| 基金项目:国家重点研发计划（2022YFD1400304）；新疆维吾尔自治区重点研发专项（2022B02033-1）；新疆维吾尔自治区“天山英才”三农骨干人才计划（2023SNGGGCC032）；新疆棉花产业技术体系（XJARS-03）；广东省基础与应用基础研究基金面上项目（2021A1515012502） |
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| 中文摘要: |
| 为明确双香豆素（DIC）对斜纹夜蛾Spodoptera litura卵巢细胞（SL-221）代谢组的影响。本研究采用CCK-8和非靶向代谢组学技术研究双香豆素抑制SL-221细胞增殖的毒理机理。结果表明，双香豆素对SL-221细胞表现出明显的抑制活性，24 h的抑制中浓度为1.60 μg/mL，且呈浓度依赖性；此外，双香豆素分别与SC 79和胰岛素共培养后，均能提升细胞活力；进一步通过非靶向代谢组学技术获得了393个差异代谢物，双香豆素与对照相比上调了364个，下调29个，而50%以上的差异代谢物分布于氨基酸及其代谢物（48.3%）和甘油磷脂类（14.2%）中；差异代谢物富集主要在氨基糖和核苷酸糖的代谢、不饱和脂肪酸的生物合成、糖的生物合成、淀粉和蔗糖等营养代谢途径中；其中，差异代谢产物肾上腺甾酮（Adrenosterone）、LPC(12:0/0:0)、N,N-二环己基碳二亚胺（N,N-dicyclohexylcarbodiimide）和L-缬氨酸-L-脯氨酸（Val-Pro）分别上调了5 833.56倍、15.47倍、10.26倍和9.94倍。本研究解析了双香豆素诱导斜纹夜蛾卵巢细胞增殖的代谢调控机制，为该化合物的开发利用提供了理论依据。 |
| 英文摘要: |
| To clarify the impact of dicoumarin (DIC) on the ovarian cells metabolome of Spodoptera litura (SL-221), this study employed CCK-8 and non-targeted metabolomics techniques to investigate the toxicological mechanism of DIC in inhibiting SL-221 cells proliferation. The results showed that DIC exhibited obvious inhibitory activity against SL-221 cells, with an inhibitory concentration of 1.60 μg/mL at 24 h, and the inhibition was concentration-dependent. Furthermore, when co-cultured with SC 79 and insulin respectively, DIC enhanced cell viability. Through non-targeted metabolomics analysis, a total of 393 differential metabolites were obtained, with 364 DIC upregulated and 29 DIC down-regulated compared to those of the control group. More than 50% differential metabolites were distributed in amino acids and their metabolites (48.3%) and glycerophospholipids (14.2%). The differential metabolites were mainly enriched in nutritional metabolic pathway of amino sugar and nucleotide sugar metabolism, unsaturated fatty acid biosynthesis, sugar biosynthesis, starch and sucrose. Among them, the differential metabolites adrenosterone, LPC (12:0/0:0), N,N-dicyclohexylcarbodiimide, and Val-Pro upregulated by 5 833.56, 15.47, 10.26, and 9.94 times, respectively. This study elucidated the metabolic regulatory mechanism of DIC-induced SL-221 cell proliferation and provided a theoretical basis for the development and utilization of this compound. |
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