《环境昆虫学报》

作者	单位
王雪芹，蒋月丽，巩中军，王恒亮，武予清	河南省农业科学院植物保护研究所，河南省农作物病虫害防治重点实验室，农业部华北南部有害生物治理重点实验室，郑州 450002

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中文摘要:

为了探讨荻草谷网蚜Sitobion miscanthi可能存在的低温应对机制，本研究选用3龄若蚜，通过转录组测序对低温处理组（-7℃，3 h，记作T）和常温对照组（22℃，3 h，记作CK）样品进行测序分析，同时利用qRT-PCR对随机选择的6个耐寒性相关基因表达量进行测定。结果显示，转录组测序、组装后共获得28 333个unigenes，在GO数据库中注释到8 843个unigenes。低温胁迫后差异表达的unigenes数量为936个，其中508个上调，428个下调。经GO功能注释和KEGG富集分析发现，差异基因（DEGs）主要集中在碳代谢、氨基酸生物合成和缬氨酸亮氨酸异亮氨酸降解通路，其中热激蛋白、海藻糖、性决定蛋白基因显著上调表达，黑芥子酶基因显著下调表达。经qRT-PCR验证，随机筛选的6个DEGs的定量结果与转录组测序结果趋势一致。综上，本研究结果为进一步挖掘与荻草谷网蚜耐寒性相关基因提供理论基础。

英文摘要:

To reveal the adaptation mechanisms of Sitobion miscanthi to low temperature, third instar larvae were analyzed by transcriptome sequencing after exposure to low temperature (-7 ℃, T group) or normal temperature (22 ℃, CK group) for three hours. Gene function annotation and related pathway enrichment analyses were performed. The expression levels of randomly selected S. miscanthi chilling resistance-related genes were also determined by quantitative real-time PCR (qRT-PCR) to verify the sequencing results. A total of 28 333 unigenes were sequenced and assembled, and 8 842 were annotated in GO. Compared with the control group, 936 unigenes were differentially expressed after low temperature exposure (508 were up-regulated and 428 were down-regulated). GO functional analysis and KEGG enrichment analysis revealed that the differentially expressed genes mainly focused on carbon metabolism, amino acid biosynthesis, and valine, leucine, and isoleucine degradation pathway. Among these genes, trehalose, heat shock protein, and sex determination protein genes were significantly up-regulated while myrosinase genes were significantly down-regulated. The qRT-PCR results for six randomly selected differentially expressed genes were consistent with the transcriptome sequencing results. This study provides a scientific basis for exploring the cold tolerance mechanism of S. miscanthi-related genes.

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