《环境昆虫学报》

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何翔宇，邵雪花，温雪梅，梁赫，秦子昕，徐玉辉，路伟,2025,宽缨酮抑制棉铃虫幼虫生长发育的差异代谢物分析[J].环境昆虫学报,(2):547-557

宽缨酮抑制棉铃虫幼虫生长发育的差异代谢物分析

Analysis of the differential metabolites of eurycomanone inhibiting the growth and development of Helicoverpa armigera larvae

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中文关键词: 宽缨酮棉铃虫生长发育代谢组学差异代谢物

英文关键词:Eurycomanone Helicoverpa armigera growth and development metabolomics differential metabolites

基金项目:国家重点研发计划（2022YFD1400304）；新疆维吾尔自治区重点研发专项（2022B02033-1）；新疆维吾尔自治区“天山英才”三农骨干人才计划（2023SNGGGCC032）；新疆棉花产业技术体系(XJARS-03)；广东省基础与应用基础研究基金面上项目（2021A1515012502）

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何翔宇，邵雪花，温雪梅，梁赫，秦子昕，徐玉辉，路伟

1. 新疆农业大学农学院，棉花教育部工程研究中心，新疆维吾尔自治区农林有害生物监测与安全防控重点实验室，乌鲁木齐 830052；2. 广东省农业科学院果树研究所，农业农村部南亚热带果树生物学与遗传资源利用重点实验室，广东省热带亚热带果树研究重点实验室，广州 510640；3. 中国农业科学院西部农业研究中心，新疆昌吉 831100

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中文摘要:

为明确宽缨酮（Eurycomanone，EN）抑制棉铃虫Helicoverpa armigera幼虫生长发育的代谢谱变化，本研究采用饲料混毒法检测了宽缨酮和糖、蛋白质及脂类对棉铃虫生长发育的影响，进一步通过非靶向代谢组学技术，对宽缨酮处理后棉铃虫幼虫的差异代谢物进行注释、筛选、分类和分析。结果表明，10 μg/g宽缨酮可显著抑制棉铃虫的生长发育，幼虫的体重降低2.32倍，蛹重降低1.35倍，化蛹率和羽化率分别降低33.82%和29.68%。添加胰蛋白胨和蔗糖可部分恢复宽缨酮的抑制活性，而添加甘油后对虫体的抑制活性无显著影响。筛选得到19个差异代谢物，其中上调11个，下调8个，主要分布在氨基酸及其代谢物、核苷酸、脂肪酰类、碳水化合物等营养代谢途径中。其中，甲磺酸、蛋氨酸亚砜、3-O-甲基多巴和3,4-二羟基苯乙酸上调较显著，与对照相比分别上调了119.80倍、3.34倍、2.40倍和2.38倍； L-蛋氨酸、十八碳二烯酰胺、L-酒石酸、腺嘌呤核苷三磷酸、胞苷-5'-三磷酸、β-D-呋喃果糖基-α-D-吡喃葡萄糖苷、香菇嘌呤和L-多巴，分别下调了0.49倍、0.48倍、0.48倍、0.41倍、0.36倍、5.41 × 10-3倍、1.30 × 10-3倍和1.48 × 10-4倍。本研究解析了宽缨酮抑制棉铃虫生长发育的代谢调控作用，为该化合物的开发利用提供了理论依据。

英文摘要:

To clarify the metabolic changes induced by Eurycomanone (EN) in inhibiting the growth and development of Helicoverpa armigera larvae. In this study, the effects of EN, as well as carbohydrates, proteins, and lipids, on the growth and development of H. armigera were examined adopting mixed toxic feed. Furthermore, non-targeted metabolomics techniques were employed to annotate, screen, classify, and analyze the differential metabolites in H. armigera larvae after EN treatment. The results of the growth and development experiment showed that 10 μg/g of EN significantly inhibited the growth and development of H. armigera larvae, with a 2.32-fold decrease in larval weight, a 1.35-fold decrease in pupal weight, and a 33.82% and 29.68% decrease in pupation rate and eclosion rate, respectively. The addition of pancreatic protein hydrolysate and sucrose partially restored the inhibitory activity of EN, while the addition of glycerol had no significant effect on inhibitory activity of larval growth and development. A total of 19 differential metabolites were screened using non-targeted metabolomics techniques, including 11 upregulated and 8 downregulated metabolites, mainly distributed in nutritional metabolic pathways related to amino acids and their metabolites, nucleotides, fatty acyls, and carbohydrates. Among them, Methanesulfonic acid, Methionine sulfoxide, 3-O-Methyldopa, and 3, 4-Dihydroxybenzeneacetic acid were upregulated more significantly, with fold changes of 119.80, 3.34, 2.40, and 2.38, respectively, compared to those of the control group. On the other hand, L-Methionine, Octadecadienamide, L-Tartaric acid, ATP, CTP, β-D-furanfructosyl-α-D-glucopyranoside, Eritadenine, and L-Dopa were down-regulated, with fold changes of 0.49, 0.48, 0.48, 0.41, 0.36, 5.41 × 10-3, 1.30 × 10-3 and 1.48 × 10-4, respectively. This study elucidated the metabolic regulatory mechanism underlying the inhibition of H. armigera growth and development by EN, providing a theoretical basis for the development and utilization of this compound.

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