| 董贤凤，杨惠棋，陈 原，谢治梅，吴渊明，刘同先，郑明辉,2025,冈田绕眼果蝇触角OBP基因家族鉴定和PoOBP83a克隆分析[J].环境昆虫学报,(2):515-525 |
| 冈田绕眼果蝇触角OBP基因家族鉴定和PoOBP83a克隆分析 |
| Identification of the OBP gene family and PoOBP83a cloning analysis in Phortica okadai antenna |
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| DOI： |
| 中文关键词: 冈田绕眼果蝇 气味结合蛋白 PoOBP83a 分子对接 |
| 英文关键词:Phortica okadai odorant binding proteins PoOBP83a molecular docking |
| 基金项目:国家自然科学基金（31960025）；贵州省科技厅技术基金（黔科合基础项目［2016］1168号）；黔南民族医学高等专科学校科研基金（Qnyz202208） |
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| 中文摘要: |
| 为了解冈田绕眼果蝇Phortica okadai触角中气味结合蛋白（OBPs）的多样性、进化关系、功能特征以及在嗅觉功能中可能发挥的作用，本研究基于冈田绕眼果蝇触角转录组测序数据进行了生物信息学分析，对转录组中的OBPs家族基因进行了鉴定和序列分析，并重点对该家族中的PoOBP83a基因进行了克隆、蛋白质理化性质、基因表达量检测以及分子对接模拟。结果表明：触角转录组中存在15个具有OBP保守结构域的PoOBPs基因，大部分PoOBPs蛋白为亲水性蛋白。PoOBPs家族的系统发育分析表明，可以将15个PoOBPs共分为5个亚家族；蛋白结构预测表明，PoOBPs均含有PBP-GOBP结构域，PoOBP83a基因编码区全长501 bp，编码166个氨基酸，蛋白分子量约19 kDa，理论等电点（pI）为6.15。PoOBP83a氨基酸序列中有6个保守半胱氨酸位点，属于Classic-C OBP家族蛋白；PoOBP83a在每个阶段均有表达，PoOBP83a在雌雄成虫触角中的相对表达量最高，其次为翅、口器和头部，在足、腹部和胸部的表达量最低。分子对接表明PoOBP83a与芳樟醇、苯甲酸乙酯、月桂酸乙酯、苯乙醇及吲哚具有较好的结合能力。本研究结果可为进一步研究冈田绕眼果蝇的嗅觉功能提供理论基础。 |
| 英文摘要: |
| The objective of this research was to explore the diversity, evolutionary relationships, functional characteristics and potential roles of the antennae odorant binding proteins (OBPs) in the olfactory function of Phortica okadai. This study conducted bioinformatics analysis based on the antennal transcriptome sequencing data of P. okadai including identification and sequence analysis of OBP family genes in the transcriptome. Special emphasis was placed on the cloning of PoOBP83a gene from this family, along with investigations of its protein physicochemical properties, gene expression profiling, and molecular docking simulations. The result showed there were 15 PoOBPs genes with OBP conserved domains in the antenna transcriptome, with the majority of PoOBP proteins exhibiting hydrophilic. These 15 PoOBPs were classified into five subfamilies based on their evolutionary relationships, which was shown in phylogenetic analysis of PoOBPs families. Protein structure predictions indicated that all PoOBP proteins possessed a PBP-GOBP domain. The coding region of the PoOBP83a gene was found to be 501 bp in length, encoding a protein of 166 amino acids, with an approximate protein molecular weight of 19 kDa and a pI of 6.15. The amino acid sequence of PoOBP83a contained six conserved cysteine sites, categorizing it within the Classic-C OBP family. Expression analysis demonstrated that PoOBP83a was present at all developmental stages, with the highest relative expression observed in antennae of male and female adults, followed by wings, mouthparts, and heads, while the lowest expression levels were recorded in legs, abdomen, and thorax. Molecular docking studies indicated that PoOBP83a exhibited strong binding affinities with linalool, ethyl benzoate, ethyl laurate, phenylethyl alcohol, and indole. The results of this investigation provide a theoretical foundation for further research into the olfactory functions of P. okadai. |
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