| 张方梅，尤伟晨，郭世保，陈俊华，史洪中，耿书宝，周 洲，乔 利,2025,茶银尺蠖触角转录组及相关嗅觉基因的分析[J].环境昆虫学报,(2):504-514 |
| 茶银尺蠖触角转录组及相关嗅觉基因的分析 |
| Analysis of the antennal transcriptome and olfaction-related genes of Scopula subpunctaria (Herrich-Schaeffer) |
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| DOI： |
| 中文关键词: 茶银尺蠖 触角转录组 嗅觉相关蛋白 基因注释 |
| 英文关键词:Scopula subpunctaria antennal transcriptome olfaction-related genes gene annotation |
| 基金项目:河南省大别山实验室重点课题（DMLP006）；河南省中央引导地方科技发展资金项目（Z20221341063）；信阳农林学院创新团队建设项目（XNKJTD-007，KJCXTD-202001）；河南省高等学校重点科研项目（24B210012），信阳生态研究院开放基金（2023XYQN08，2023XYMS11）；河南省国际科技合作项目（242102520042）；河南省科技攻关项目（242102110178） |
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| 中文摘要: |
| 本研究利用IIIumina Hiseq 4000高通量测序平台对茶银尺蠖Scopula subpunctaria（Herrich-Schaeffer）雌雄成虫触角进行转录组测序，共获得93 335条unigenes，N50长度为1 151 bp。通过基因功能注释，有23 581条unigenes注释到六大公共数据库中，其中注释到NR数据库的unigenes最多，占39.96%，且与家蚕Bombyx mori相似基因数目最多，占20.32%；10 718条unigenes注释到GO数据库的生物过程、细胞组分和分子功能3个大类46个亚类；5 464条unigenes注释到KEGG数据库的38个小类代谢通路，其中注释在信号传导通路的unigenes最多，占10.64%。根据注释信息，共筛选得到60个候选嗅觉基因，包含17个气味结合蛋白基因（Odorant binding protein genes，OBPs）、13个化学感受蛋白基因（Chemosensory protein genes，CSPs）、14个嗅觉受体基因（Olfactory receptor genes，ORs）、13个离子型受体基因（Ionotropic receptor genes，IRs）和3个感觉神经膜蛋白基因（Sensory neuron membrane protein genes，SNMPs）。利用FPKM值对SsubOBPs和SsubCSPs基因进行表达量分析发现，SsubOBP2和SsubOBP3在茶银尺蠖雌雄成虫触角中均高丰度表达（FPKM>2 000），SsubCSP4、SsubCSP7和SsubCSP9在茶银尺蠖雌雄成虫触角中均高丰度表达（FPKM>900）。本研究首次获得了茶银尺蠖触角转录组数据，并鉴定出候选嗅觉相关基因，结果为进一步研究茶银尺蠖的嗅觉感受机制提供分子基础。 |
| 英文摘要: |
| In this study, the transcriptome of male and female adult antennae of Scopula subpunctaria was sequenced using Illumina HiSeq 4 000 platform. A total of 93 335 unigenes with N50 length of 1 151 bp were obtained. After annotating for unigenes in gene function database, 23 581 unigenes were annotated in six databases. Of these, 39.96% unigenes were annotated in the NR database, which exhibited the highest similarity (20.32%) to those of Bombyx mori. A total of 10 718 unigenes were annotated in the GO database, which were categorized into three groups (biological process, cellular components and molecular function) and 46 subcategories. In the KEGG database, 5 464 unigenes were assigned to 38 known metabolic pathways of subcategories. Among these, 10.64% unigenes were involved in the signal transduction pathway and it was the highest. Based on the annotation information, 60 olfaction-related genes fom S. subpunctaria including 17 odorant binding protein genes (OBPs), 13 chemosensory protein genes (CSPs), 14 olfactory receptor genes (ORs), 13 ionotropic receptor genes (IRs) and 3 sensory neuron membrane protein genes (SNMPs) were obtained. The FPKM value was utilized to analyze the gene expression of SsubOBPs and SsubCSPs. The findings indicated that the gene expression of SsubOBP2 and SsubOBP3 were highly expressed in the adult antennae of S. subpunctaria females and males (FPKM>2 000), and SsubCSP4, SsubCSP7 and SsubCSP9 were expressed with high abundance in the adult antennae of both sexes (FPKM>900). This study obtained the antennal transcriptome data of S. subpunctaria for the first time, and identified olfaction-related genes, providing a molecular foundation for further research into the olfactory mechanisms of S. subpunctaria. |
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