| 陈嘉明，罗丽丹，安 琪，刘 磊，孙勤哲，刘 欢，王森山，宋丽雯,,3种杀螨剂胁迫对截形叶螨TtABCB和TtABCG基因表达的影响[J].环境昆虫学报,(): |
| 3种杀螨剂胁迫对截形叶螨TtABCB和TtABCG基因表达的影响 |
| Effects of exposure to three acaricides on the expression of TtABCB and TtABCG genes in Tetranychus truncatus |
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| DOI： |
| 中文关键词: 截形叶螨 ABC转运蛋白 杀螨剂胁迫 表达机制 |
| 英文关键词:Tetranychus truncatus ATP-binding cassette transporter exposure to acaricide expression mechanism |
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| 摘要点击次数: 15 |
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| 中文摘要: |
| 截形叶螨Tetranychus truncatus是玉米等农作物上主要害螨之一，现已对许多药剂产生抗性，导致防治难度加大。ABC转运蛋白（ATP-binding cassette transporter）作为一种广泛存在于各种生物体内的跨膜超蛋白家族，可通过内、外源底物的跨膜运输在对药剂的解毒代谢方面发挥重要作用。本研究对截形叶螨ABC转运蛋白基因进行了筛选及系统发育分析，测定并比较了阿维菌素、炔螨特和联苯肼酯在不同时间处理下对叶螨的毒力，并通过RT-qPCR技术探究截形叶螨雌成螨ABC转运蛋白两个亚家族基因TtABCB和TtABCG在叶螨不同发育阶段的表达及对阿维菌素、炔螨特和联苯肼酯3种杀螨剂胁迫时的响应。结果表明，根据截形叶螨转录组数据共筛选出62个ABC转运蛋白基因，经系统发育和保守结构域分析，这些基因属于8个亚家族，命名为TtABCA ~ TtABCH，其中TtABCB和TtABCG亚家族在截形叶螨各发育阶段（卵、幼螨、若螨和成螨）均有表达，但不同发育阶段相对表达量有所不同；3种药剂处理截形叶螨雌成螨24 h和48 h后，毒力结果为阿维菌素 > 联苯肼酯 > 炔螨特；处理72 h后，3种药剂对截形叶螨雌成螨毒力有所变化，最高为阿维菌素，其次是炔螨特，毒力最低的为联苯肼酯。TtABCB1、TtABCB3、TtABCB4和TtABCG1等4个基因在阿维菌素、炔螨特和联苯肼酯胁迫下，表达量显著上调，表明截形叶螨可能通过上调这4个基因来应对阿维菌素、炔螨特和联苯肼酯的胁迫，其中TtABCB3和TtABCG1基因可能参与截形叶螨对3种杀螨剂的解毒代谢。该结论为截形叶螨靶标抗性基因筛选和综合治理提供了理论依据。 |
| 英文摘要: |
| Tetranychus truncatus is one of the major pests on maize and other crops, and it has developed resistance to numerous pesticides, which makes its control more difficult. ATP-binding cassette (ABC) transporters, a ubiquitous transmembrane protein superfamily in organisms, play crucial roles in detoxification by mediating the transmembrane transport of endogenous and exogenous substrates. In this study, we systematically screened ABC transporter genes of T. truncatus and made a phylogenetic analyses. In addition, the toxicity of abamectin, propargite and bifenazate to T. truncatus at different exposure durations was evaluated. RT-qPCR was used to evaluate the expression profiles of two ABC transporter subfamilies, TtABCB and TtABCG, at different developmental stages of female adults (eggs, larvae, nymphs and adults) and their transcription responses to the three acaricides. The results showed that 62 ABC transporter genes were identified from the transcriptome data of T. truncatus. According to phylogenetic analysis and conserved domain analysis, these genes were classified into eight subfamilies(TtABCA~TtABCH). Among them, TtABCB and TtABCG subfamilies were expressed at all developmental stages of T. truncatus (egg, larva, nymph, and adult), but the relative expression levels were different at developmental stages. Toxicity test showed that abamectin had the highest efficacy against female adults at 24 hours and 48 hours (toxicity ranking was abamectin > bifenazate > propargite.)，while at 72 hours, the toxicity of propargite was higher than that of bifenazate (abamectin > propargite > bifenazate). Notably, four genes- TtABCB1, TtABCB3, TtABCB4 and TtABCG1 4 were significantly up-regulated when exposed to all three acaricides, indicating their potential role in reducing chemical stress. Further analysis suggested that TtABCB3 and TtABCG1 may be involved in the detoxification metabolism of T. truncatus to the three acaricides. These findings promote our understanding of the resistance mechanisms of T. truncatus, and provide important insights for screening targeted resistance genes and integrated pest management. |
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