| 李咏祺，丁文兵，贺华良，高 俏，高宏帅，李有志，邱 林,,白背飞虱致病细菌Acinetobacter soli的分离鉴定及其杀虫活性测定[J].环境昆虫学报,(): |
| 白背飞虱致病细菌Acinetobacter soli的分离鉴定及其杀虫活性测定 |
| Isolation and identification of a pathogenic bacterium Acinetobacter soli from Sogatella furcifera, and determination of its insecticidal activity |
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| DOI： |
| 中文关键词: 白背飞虱 病原微生物 单不动杆菌 杀虫活性 |
| 英文关键词:Sogatella furcifera pathogenic microbes Acinetobacter soli insecticidal activity |
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| 摘要点击次数: 12 |
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| 中文摘要: |
| 白背飞虱Sogatella furcifera是重要的水稻害虫，通过取食和传播病毒病对水稻造成严重的危害。因此，开发和利用病原微生物防治白背飞虱具有重要现实意义。本研究从白背飞虱体内分离出1株细菌，命名为HUNAN-1。通过形态学及分子生物学手段对该菌株进行了鉴定，并采用喷雾法测定了该菌株菌液对白背飞虱的室内杀虫活性，同时评估了培养时间和培养基pH值对发酵上清液杀虫活性的影响。结果表明，菌株HUNAN-1为不动杆菌属，单不动杆菌Acinetobacter soli。菌株HUNAN-1对白背飞虱表现出显著的杀虫活性。当浓度为109 CFU/mL时，菌液对白背飞虱的校正死亡率达到100%，其LT50为31.523 h，LC50值为2.71 × 106 CFU/mL。菌株HUNAN-1的发酵上清液同样对白背飞虱有较高的致死率，处理72 h后，白背飞虱的死亡率超过80%，并且不同发酵时间和培养基pH值对发酵上清液的杀虫活性无显著影响。综上，菌株HUNAN-1对白背飞虱具有良好的防治潜力，应用前景广阔。 |
| 英文摘要: |
| The white-backed planthopper (Sogatella furcifera) is a significant pest of rice, inflicting severe damage to crops through feeding and transmitting of viral diseases. Therefore, developing and utilizing pathogenic microorganisms for the management of S. furcifera holds substantial practical importance. In this study, a bacterial strain, designated as HUNAN-1, was isolated from S. furcifera. The strain was identified using morphological and molecular biological approaches, and its insecticidal activity, as well as the influence of fermentation time and pH value on the insecticidal activity of the fermentation supernatant, were assessed through a spray method. The results revealed that strain HUNAN-1 has the genus name Acinetobacter and species name Acinetobacter soli. This strain exhibited notable insecticidal effects against S. furcifera, achieving a cumulative corrected mortality rate of 100% at a concentration of 109 CFU/mL, with an LT50 value of 31.523 hours. Additionally, the LC50 value was determined to be 2.71 × 106 CFU/mL. The fermentation supernatant of strain HUNAN-1 demonstrated high lethality against S. furcifera, with mortality rates exceeding 80% after 72 hours of treatment. Notably, variations in fermentation duration and medium pH had no significant impact on the insecticidal activity of fermentation supernatant. In conclusion, strain HUNAN-1 shows considerable control potential of S. furcifera, with broad application prospects. |
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