| 廖 钢，周忠林，向婷婷，陈亚平，桂富荣，孙仲享,2025,基于生物信息学的番茄潜叶蛾味觉受体基因家族特征及发育表达探究[J].环境昆虫学报,(1):66-75 |
| 基于生物信息学的番茄潜叶蛾味觉受体基因家族特征及发育表达探究 |
| Identification of the gustatory receptor gene family of Tuta absoluta and its expression analysis at different developmental stages |
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| DOI： |
| 中文关键词: 番茄潜叶蛾 味觉受体 基因家族 系统发育 生物信息学分析 |
| 英文关键词:Tuta absoluta gustatory receptor gene family phylogeny bioinformatics analysis |
| 基金项目:国家重点研发计划（2021YFD1400200）；云南省科技厅资助项目（202301AT070485） |
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| 中文摘要: |
| 昆虫的味觉受体是一类关键的化学感受器，能够探测外部环境的刺激，并对昆虫的寄主选择、摄食、交配和产卵等行为产生影响。本研究以重大入侵害虫番茄潜叶蛾Tuta absoluta（Meyrick）为研究对象，为明确番茄潜叶蛾味觉受体基因家族（Gustatory Receptor，GR）特征和探讨番茄潜叶蛾不同发育阶段GR家族基因的表达情况，利用生物信息学方法对番茄潜叶蛾GR基因进行鉴定、染色体定位、系统发育分析及龄期表达分析。结果表明，鉴定出番茄潜叶蛾GR基因62个，且分布于19条染色体上，编码蛋白质序列长度为318~2 034 bp，平均外显子（Exon）片段数量为5个，具有1~7个跨膜区。系统发育分析表明，番茄潜叶蛾GR基因可分为4个亚家族，包括苦味受体（35个）、二氧化碳受体（1个）、糖受体（5个）和Gr43a-like受体（7个），其余基因则未分类。不同发育阶段的表达分析表明，番茄潜叶蛾GR基因在不同发育阶段的表达存在显著差异。研究结果为进一步明确番茄潜叶蛾味觉受体基因功能，揭示其与寄主植物的协同进化关系奠定了基础，这些发现为利用功能植物来控制番茄潜叶蛾提供了科学依据，并为从味觉受体角度开发新的害虫防控技术奠定基础。 |
| 英文摘要: |
| Insect gustatory receptors are a crucial class of chemoreceptors that can detect external environmental stimuli and influence insect behaviors such as host selection, feeding, mating, and oviposition. This study focused on the invasive pest Tuta absoluta (Meyrick) that affected tomatoes. To elucidate the characteristics of the gustatory receptor (GR) gene family in T. absoluta and investigate the expression patterns of GR genes across different developmental stages, we employed bioinformatics approaches to identify, chromosomally locate, conduct phylogenetic analysis, and analyze age-stage expression profiles of the GR genes in T. absoluta. The results indicated that 62 GR genes of the T. absoluta moth were identified and distributed on 19 chromosomes. The length of the encoded protein sequence ranged from 318 to 2034 bp, with an average of 5 exon fragments and 1 to 7 transmembrane regions. Phylogenetic analysis revealed that the GR genes of the T. absoluta could be categorized into four distinct subfamilies: bitter taste receptors (35 genes), carbon dioxide receptors (1 gene), sugar receptors (5 genes), and Gr43a-like receptors (7 genes). The remaining genes have not been classified yet. Expression analysis across different developmental stages revealed that the GR genes of the T. absoluta exhibited significant variation in expression levels throughout its life cycle. The research results provided a solid foundation for further elucidating the functions of gustatory receptor genes in the T. absoluta and uncovering its co-evolutionary relationship with host plants. These findings offered scientific evidence for controlling the T. absoluta by functional plants and lay the foundation for developing novel pest control technologies from a gustatory receptor perspective. |
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