| 朱芷莹，林志凯，陈思博，王淳静，肖妍虹，余小强，胡启豪,,Kcmf1基因在家蚕和斜纹夜蛾精巢中的表达及功能初探[J].环境昆虫学报,(): |
| Kcmf1基因在家蚕和斜纹夜蛾精巢中的表达及功能初探 |
| Expression and functional analysis of Kcmf1 gene in the testis of Bombyx mori and Spodoptera litura |
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| DOI： |
| 中文关键词: Kcmf1 精巢 精子发生 家蚕 斜纹夜蛾 |
| 英文关键词:Kcmf1 testis spermatogenesis Bombyx mori Spodoptera litura |
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| 中文摘要: |
| Kcmf1基因属于E3泛素化酶家族，在哺乳动物器官发育及癌症发生过程中发挥作用，但在哺乳动物及昆虫生殖系统中的功能仍不清楚。本研究通过PCR实验获得了家蚕Bombyx mori BmKcmf1（1 110 bp）和斜纹夜蛾Spodoptera litura SlKcmf1（966 bp）两个基因的ORF。生物信息学分析结果显示，BmKCMF1和SlKCMF1蛋白均包含保守的锌结合域与C2H2型锌指结构域。qRT-PCR结果显示，BmKcmf1和SlKcmf1在不同发育阶段的家蚕和斜纹夜蛾精巢中均有表达，且它们的表达随着发育时期而变化，提示BmKcmf1和SlKcmf1的功能与精巢发育有关。在家蚕BmN和斜纹夜蛾Sl221细胞株中分别过表达BmKCMF1和SlKCMF1蛋白，结果发现Vasa、Hop、Dpp等精子发生关键基因的表达发生显著变化。由此，本研究推测BmKcmf1和SlKcmf1基因参与家蚕和斜纹夜蛾精巢发育及精子发生等过程。 |
| 英文摘要: |
| The Kcmf1 gene belongs to the E3 ubiquitin ligase family and is related to the organ development and oncogenesis in mammals. However, the function of Kcmf1 in the reproductive systems of mammals and insects remains unclear. In this paper, we obtained the ORFs of Bombyx mori BmKcmf1 (1 110 bp) and Spodoptera litura SlKcmf1 (966 bp) through PCR method. Bioinformatics analysis showed that both BmKCMF1 and SlKCMF1 proteins contain conserved zinc-binding domains and C2H2 type zinc finger domains. Moreover, we found that BmKcmf1 and SlKcmf1 were expressed in the testis of B. mori and S. litura at different developmental stages by real-time quantitative PCR (qRT-PCR), suggesting that BmKcmf1 and SlKcmf1 may be related to testis development. Furthermore, when BmKCMF1 and SlKCMF1 were overexpressed in the BmN cells and Sl221 cells, respectively, the mRNA levels of genes related to spermatogenesis such as Vasa, Hop and Dpp changed significantly. In conclusion, our results suggest that BmKcmf1 and SlKcmf1 genes might participate in the spermatogenesis of B. mori and S. litura. |
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