| 哈帕孜·恰合班，张鹏飞，郭家洛，代丽珍，王进忠，吐尔逊·那吾尔孜拜，任争光，武延生,,为害新疆春小麦的黑角负泥虫形态鉴定与COⅠ基因序列分析[J].环境昆虫学报,(): |
| 为害新疆春小麦的黑角负泥虫形态鉴定与COⅠ基因序列分析 |
| Morphological identification and COⅠ gene sequence analysis of the cereal leaf beetle Oulema melanopus (Chrysomelidae: Criocerinae) infesting spring wheat in Xinjiang, China |
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| DOI： |
| 中文关键词: 新疆青河县 负泥虫 外部形态 生殖器 COⅠ基因序列分析 |
| 英文关键词:Qinghe county cereal leaf beetle external morphology genitalia COI gene sequence analysis |
| 基金项目: |
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| 摘要点击次数: 4 |
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| 中文摘要: |
| 为了明确来自中国新疆青河县危害春小麦的一种负泥虫的种类，对其成虫外部形态和生殖器官结构进行了观察，并分析了其线粒体COⅠ基因序列和单倍型。结果显示，成虫体长4.98~5.61 mm，头部深蓝色，触角黑色11节，前胸背板橙红色。鞘翅长3.44~3.67 mm，每侧具10列由密而深刻点组成的条纹。腿节（除基部）、胫节（除端部）为橙红色，其余为黑色。雄性阳茎内的插入器（鞭毛）粗壮略短，长518.99 μm，宽86.29 μm，轻微弯曲。雌性受精囊导管在近端部硬化严重，长496.95 μm，盘曲折叠1圈。这些外部形态特征和生殖器结构与黑角负泥虫Oulema melanopus相符。线粒体COⅠ序列分析结果显示，与来自其他国家的11只黑角负泥虫COⅠ序列的遗传距离范围为0~0.091，系统发育表明与黑角负泥虫、O. mauroi和O.duftschmidi的亲缘关系最近，单倍型分析结果显示，本序列单独属于一个单倍型。 |
| 英文摘要: |
| In order to clarify the species of a cereal leaf beetle feed on spring wheat in Qinghe County, Xinjiang, China, the external morphology and internal reproductive organ structure of their adults were observed, and the mitochondrial COⅠ gene sequence and haplotype were also analyzed. The results showed that the body length of adult was 4.98~5.61 mm and head was dark blue. Antennae was black, 11-segmented and pronotum was orange-red. Elytra was 3.44~3.67 mm in length, covered with 10 striae composed of dense and deep punctures. The coxae, trochanters, tarsi and apices of tibiae are black, femora and tibiae are orange. The intromittent organ (flagellum) in male aedeagus is short, stout, and only slightly curved, with a length of 518.99 μm and a width of 86.29 μm. The ductus spermathecae is approximately 496.95 μm in length, with a heavily sclerotized proximal part, and forms one simple coil. These external morphological features and genital structures are consistent with Oulema melanopus. Mitochondrial COI sequence analysis showed that the genetic distances between the 11 O. melanopus from other countries ranged from 0~0.091, and the phylogeny also indicated that O. melanopus, O. mauroi, and O. duftschmidi were closest affinities. Haplotype analysis showed that it belong to one haplotype alone. |
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