《环境昆虫学报》

鉴定分析梨星毛虫Illiberis pruni性信息素合成酶基因，为探索其性信息素合成分子机制提供理论依据。基于梨星毛虫雌雄腹部及雌翅转录组数据，筛选鉴定性信息素合成酶基因并进行生物信息学分析，利用实时荧光定量PCR分析其在雌雄成虫不同组织中的表达情况。共鉴定出21个性信息素合成酶基因（3个Dess、2个FARs、1个FAS、10个ACTs、3个ACCs和2个ARs），生物信息学分析发现这些基因蛋白质分子质量差异较大，属于不稳定蛋白；亚细胞定位显示其蛋白主要位于细胞质和线粒体中；系统发育树结果推测梨星毛虫ACCs基因和ACTs基因与小线角木蠹蛾和黑脉金斑蝶的性信息素相关基因亲缘关系较近；通过RT-qPCR分析表明16个基因表现为雌性翅偏向表达，7个基因在雌性腹部高表达，2个基因在雄性胸部偏向表达。研究结果为梨星毛虫性信息素相关基因功能研究奠定基础。

英文摘要:

The identification and analysis of sex pheromone-related genes in Illiberis pruni provided a theoretical basis for exploring the molecular mechanisms of sex pheromone synthesis. Based on transcriptome data from the abdomen and female wings of I. pruni, sex pheromone-related genes were screened and identified followed by bioinformatics analysis. Real-time fluorescence quantitative PCR was used to analyze the expression patterns of these genes in different tissues of male and female adults. A total of 21 sex pheromone-related genes were identified (3 Dess, 2 FARs, 1 FAS, 10 ACTs, 3 ACCs and 2 ARs), and bioinformatics analysis revealed that these genes had significant variation in protein molecular weight, suggesting that they were unstable proteins. Subcellular localization analysis showed that the corresponding proteins were mainly located in the cytoplasm and mitochondria. Based on the phylogenetic tree results, it was inferred that the ACCs genes and ACTs genes of I. pruni were closely related to the sex pheromone-related genes of Streltzoviella insularis and Danaus plexippus. RT-qPCR analysis demonstrated that 16 genes exhibited female-biased expression in the wings, 7 genes were highly expressed in the female abdomen, and 2 genes showed male-biased expression in the male thorax. These research findings provided a foundation for the functional study of sex pheromone-related genes in I. pruni.

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