| 陈宜明，戴阳朔，巩 固，袁龙宇，李燕芳，许益镌，张振飞,,水稻抗虫次生化合物麦黄酮刺激对褐飞虱唾液腺转录组的影响[J].环境昆虫学报,(): |
| 水稻抗虫次生化合物麦黄酮刺激对褐飞虱唾液腺转录组的影响 |
| Effects of tricin stimulation on salivary gland transcriptome of brown planthopper Nilaparvata lugens (Stål) |
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| DOI： |
| 中文关键词: 唾液腺基因 麦黄酮刺激 褐飞虱 转录组 |
| 英文关键词:Salivary gland genes tricin stimulation BPH transcriptome |
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| 摘要点击次数: 50 |
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| 中文摘要: |
| 为深入探究褐飞虱Nilaparvata lugens如何应答水稻抗虫物质麦黄酮的刺激，并揭示其背后的转录调控机制，本研究采用转录组学方法，系统分析了在麦黄酮刺激下褐飞虱唾液腺组织的转录水平变化。研究结果显示，与空白对照相比，褐飞虱唾液腺在受到麦黄酮处理6 h和24 h后的差异表达基因分别为49个和232个，两个时间段共同差异基因为23个。长时间的麦黄酮处理导致的差异基因数量显著高于短期处理，且这些基因的表达量也呈现随着时间延长而增加的趋势。进一步通过GO功能注释分析发现，这些共同差异基因主要参与刺激响应和胁迫响应等生物进程。同时，KEGG分析发现这些基因主要集中在蛋白质消化吸收和内分泌调节等相关途径。共同差异表达基因的RT-qPCR分析证实麦黄酮刺激确实能诱导褐飞虱唾液腺中胰蛋白酶、麦芽糖酶和转运蛋白相关基因的表达。这些结果将有助于揭示褐飞虱应对麦黄酮刺激的分子机制，更是说明胰蛋白酶和有机阴离子转运蛋白LOC111045154可能成为未来防治褐飞虱的潜在靶点。 |
| 英文摘要: |
| In order to investigate how brown planthopper (BPH, Nilaparvata lugens) responds to the stimulation of tricin, an anti-insect substance in rice, and reveal the transcriptional regulation mechanism behind it, this study systematically analyzed the transcriptional changes in salivary gland tissue of BPH under the stimulation of tricin using transcriptomic methods. The results showed that compared with the blank control, 49 and 232 differentially expressed genes were found in the salivary glands of BPH treated with tricin for 6 h and 24 h, respectively, and 23 common differentially expressed genes were found in the salivary glands of BPH. The number of differential genes caused by long-term treatment of tricin was significantly higher than that caused by short-term treatment, and the expression of these genes also showed a trend of increasing with the extension of time. Further analysis by GO functional annotation showed that these common differential genes were mainly involved in biological processes such as response to stimulus and response to stress. At the same time, KEGG analysis found that these genes were mainly concentrated in protein digestion and absorption and pancreatic secretions and other related pathways. RT-qPCR analysis of common differentially expressed genes confirmed that tricin stimulation could indeed induce the expression of trypsin, maltase and transporter-related genes in the salivary glands of BPH. These results will help to reveal the molecular mechanism of BPH response to tricin stimulation, and also indicate that trypsin and organic anion transporter LOC111045154 may be potential targets for future control of BPH. |
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