| 吕 军，程 琴，李 超，康 奎,,南方水稻黑条矮缩病毒对白背飞虱内共生菌群落的影响[J].环境昆虫学报,(): |
| 南方水稻黑条矮缩病毒对白背飞虱内共生菌群落的影响 |
| The impact of Southern rice black-streaked dwarf virus (SRBSDV) on commensal bacteria in White-Backed Planthoppers (WBPH) |
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| DOI： |
| 中文关键词: 南方水稻黑条矮缩病毒 白背飞虱 水稻 16S rDNA 共生菌 |
| 英文关键词:SRBSDV Sogatella furcifera rice 16S rDNA commensal bacteria |
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| 中文摘要: |
| 南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus，SRBSDV）是一种仅通过白背飞虱Sogatella furcifera介导传播的病毒，造成了严重的水稻产量损失。白背飞虱作为唯一的传播媒介，其携带SRBSDV后对体内共生菌的影响尚不清楚。为探究SRBSDV对白背飞虱内共生菌群落结构的影响，本研究首先利用感染SRBSDV的水稻饲养白背飞虱，并通过特异性引物扩增测序方法确认飞虱是否携带病毒。随后采用16S rDNA测序技术对飞虱体内的共生菌进行了深入分析。结果显示，白背飞虱体内的共生菌包含34个分类单元，涵盖19个不同的种属。尽管携带SRBSDV并未显著影响共生菌的物种丰富度，但感染后共生菌的物种均匀度出现了显著下降。此外，未感染SRBSDV的白背飞虱中，Wolbachia和Cardinium是主要的内生菌。然而，在感染SRBSDV后，Cardinium的相对含量显著减少，而Wolbachia、Stenotrophomonas、Thauera和Pseudonocardia的相对含量则显著增加。特别是Wolbachia，其在内生菌中成为了优势种群。这些发现为理解SRBSDV如何影响白背飞虱内共生菌的群落结构提供了重要信息，并可能对揭示SRBSDV传播的生态学机制有所助益，为进一步的研究提供了新的视角。 |
| 英文摘要: |
| The Southern rice black-streaked dwarf virus (SRBSDV) is a virus that is transmitted solely through the white-backed planthopper (WBPH), causing severe losses in rice production. As the sole vector for SRBSDV transmission, the impact of the virus on the commensal bacteria within the WBPH remains unclear. To explore the impact of SRBSDV on the structure of the commensal bacterial community within the WBPH, this study first used rice infected with SRBSDV to feed the WBPH and confirmed the presence of the virus in the WBPH using a specific primer amplification sequencing method. Subsequently, 16S rDNA sequencing technique was employed for an in-depth analysis of the symbiotic bacteria within the WBPH. The results showed that the symbiotic bacteria within the WBPH included 34 taxonomic units, encompassing 19 different genera. Although carrying SRBSDV did not significantly affect the species richness of the symbiotic bacteria, the species evenness of the symbiotic bacteria significantly decreased after infection. In addition, in the WBPH not infected with SRBSDV, Wolbachia and Cardinium were the main commensal bacteria. However, after infection with SRBSDV, the relative abundance of Cardinium significantly decreased, while the relative abundance of Wolbachia, Stenotrophomonas, Thauera, and Pseudonocardia significantly increased. In particular, Wolbachia became the dominant group among the commensal bacteria. These findings provide important information for understanding how SRBSDV affects the community structure of commensal bacteria within the WBPH and may contribute to revealing the ecological mechanisms of SRBSDV transmission, offering a new perspective for further research. |
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