《环境昆虫学报》

为了明确暗黑鳃金龟Holotrichia parallela成虫趋性行为与梨树挥发物的相关性，解析暗黑鳃金龟成虫嗅觉偏好行为。基于前期暗黑鳃金龟成虫在梨树+核桃+蓖麻+甘薯的生态系统中偏好趋向梨树的研究结果，利用气相色谱-质谱联用仪（GC-MS）测定梨树挥发物，利用气相色谱-触角电位联用仪（GC-EAD）测定暗黑鳃金龟雌雄成虫触角对梨树挥发物的电位反应，采用嗅觉行为装置和罩笼试验分别测定暗黑鳃金龟雌雄成虫对梨树主要挥发物的趋性。试验结果表明，梨树挥发物的主要成分为(E)-β-罗勒烯、(3E)-4,8-二甲基-1,3,7-壬三烯（DMNT）、乙酸-顺-3-己烯酯、2-乙基己醇、芳樟醇和α-法尼烯。暗黑鳃金龟雌雄成虫触角对梨树挥发物(E)-β-罗勒烯、DMNT、2-乙基己醇、α-法尼烯产生强电位反应，对芳樟醇、乙酸-顺-3-己烯酯产生弱电位反应。雌虫对(E)-β-罗勒烯和α-法尼烯有显著趋性，雄虫对(E)-β-罗勒烯、2-乙基己醇和α-法尼烯有显著趋性。这一研究结果为利用引诱剂有效控制暗黑鳃金龟成虫提供了理论依据。

英文摘要:

In order to clarify the correlation between the behavioral preference of Holotrichia parallela adult and plant volatiles, and to analyze the olfactory recognition of the adult. Based on the result H. parallela adults prefer to feeding and mating towards pear in the ecosystem of pear + walnut + castor + sweetpotato in the previous studies. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the main components of volatiles in pear plants. Gas chromatography-electroantennogram (GC-EAD) was used to evaluation the electrophysiological responses of the antennae of H. parallela to pear plant volatiles. The preferences of H. parallela adults to different volatiles were assessed in the binary-choice tube and artificial cage. The results showed that main compounds from the volatiles in pear plants are (E)-β-ocimene, (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), (Z)-3-hexenyl acetate, 2-ethyl-1-hexanol, linalool and α-farnesene. The GC-EAD experiment showed the relative EAG response values of male and female of H. parallela to pear plant volatiles (E)-β-ocimene, DMNT, 2-ethyl-1-hexanol, and α-farnesene were higher while those were lower to Linalool and (Z)-3-hexenyl acetate. The female of H. parallela exhibited significant preference tendency to (E)-β-ocimene and α-farnesene while the male showed the significant preference tendency to (E)-β-ocimene, 2-ethyl-1-hexanol and α-farnesene. These research findings provide a theoretical basis for the effective control of H. parallela adult using attractants.

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