| 寇桂香，陈 帅，来有鹏，何啟月，牛 烁，周渊涛,,梨星毛虫不同发育阶段转录组及差异表达基因分析[J].环境昆虫学报,(): |
| 梨星毛虫不同发育阶段转录组及差异表达基因分析 |
| Transcriptome and differential gene expression analyses of different developmental stages of the Illiberis pruni |
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| DOI： |
| 中文关键词: 梨星毛虫 转录组 生长发育 差异表达基因 |
| 英文关键词:Illiberis pruni transcriptome growth and development differential expression gene |
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| 摘要点击次数: 40 |
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| 中文摘要: |
| 本研究基于转录组学对梨星毛虫Illiberis pruni不同生长发育阶段的差异表达基因（DEGs）进行筛选和功能注释分析，揭示与梨星毛虫生长发育相关的关键基因和信号通路。采用高通量测序技术Illumina HiSeqTM 2000检测梨星毛虫幼虫、蛹、成虫转录组差异，并对测序后筛选出的差异表达基因进行实时荧光定量PCR（RT-qPCR）验证。结果表明，转录组数据共获得80 528条Unigene，其中长度在1 kb以上的Unigene有21 743条，25 643条Unigene从七大数据库中得到注释，筛选出12个梨星毛虫不同生长发育阶段的差异表达基因，且RNA-Seq测序结果与RT-qPCR验证结果一致。研究结果将扩充梨星毛虫生长发育基因数据库信息，进一步为其在分子水平上的研究提供数据支撑。 |
| 英文摘要: |
| The purpose of this study was to analyze the differences in transcriptome data from the transcriptome level, and filter out the differential expressed genes in different growth and development stages of the Illiberis pruni. High-throughput sequencing technology Illumina HiSeqTM 2000 was used to detect the transcriptome differences in larvae, pupae and adults of the I. pruni, and the differentially expressed genes after sequencing were verified by real-time fluorescence quantitative PCR (RT-qPCR). The results showed that a total of 80 528 Unigenes were obtained from the transcriptome data, among which 21 743 Unigene with a length of more than 1 kb were obtained, 25 643 Unigenes were annotated in the seven major databases, and 12 differential genes of different growth and development stages were screened. The results of RNA-Seq sequencing were consistent with those RT-qPCR. The results of this study will expand the growth and development gene database of the I. pruni, and further provide data support for its research at the molecular level. |
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