《环境昆虫学报》

喀什是石榴的重要产区，经调查发现如今细须螨Tenuipalpus hornotinus是危害该地区石榴的优势害螨，查阅文献得知，目前针对该螨仅依赖形态学进行鉴定，关于其DNA条形码还未见报道。但该螨体积微小，相似种较多，形态学鉴定较为困难，因此本研究利用形态学结合DNA条形码技术实现该螨的快速准确鉴定。本研究通过实地观察、采集样本、制作标本和显微镜观察的方式，结合形态学资料对该螨进行形态特征比对、鉴定；同时，利用DNA条形码技术扩增COI、ITS2、28S-rRNA等基因并测序，对获得的序列进行分析，并通过MEGA7.0软件构建系统进化树。经形态学特征鉴定为如今细须螨，利用DNA条形码技术获得了该害螨的条形码序列。通过形态学和DNA条形码技术实现了石榴如今细须螨快速准确鉴定，为后续该害螨的防控提供物种基础。

英文摘要:

Kashi is an important production area of Punica granatum L., investigation found that Tenuipalpus hornotinus is the dominant pest mite that harms P. granatum L. in the region. Upon literature review, it was found that identification only relies on morphology, and there has been no report on the DNA barcode of the mite. Due to its small size and numerous similar species, morphological identification of this mite is difficult. Therefore, this study utilizes morphology combined with DNA barcode technology to achieve rapid and accurate identification of this mite. This study conducted on-site observations, collected samples, and made specimens; observing under the microscope combined with morphological data, and comparing and identifying morphological features; meanwhile, genes such as COⅠ, ITS2 and 28S-rRNA were amplified and sequenced using DNA barcode technology, analyze the obtained sequences and construct a phylogenetic tree using MEGA7.0 software. Identified by morphological features as T. hornotinus. Barcode sequence of the pest mite was obtained using DNA barcode technology. Rapid and accurate identification of T. hornotinus was achieved through morphology and DNA barcode technology, providing a species basis for the subsequent prevention and control of this pest mite.

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