| 张泓，李志强，贾凤龙，陈伟文,2024,miR-120对台湾乳白蚁抵御绿僵菌的免疫反应的影响[J].环境昆虫学报,(4):948-957 |
| miR-120对台湾乳白蚁抵御绿僵菌的免疫反应的影响 |
| Impact of miR-120 on the immune response of Coptotermes formosanus against Metarhizium anisopliae |
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| DOI： |
| 中文关键词: 台湾乳白蚁 miR-120 免疫反应 绿僵菌 白蚁防治 |
| 英文关键词:Coptotermes formosanus miR-120 immune response Metarhizium anisopliae termite control |
| 基金项目:广州市科技重点项目（201904020002）；广州市科技项目（2023A04J0147） |
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| 中文摘要: |
| 本研究探究了miR-120在台湾乳白蚁Coptotermes formosanus抵御绿僵菌Metarhizium anisopliae的免疫反应中的作用。研究发现通过对台湾乳白蚁注射miR-120 agomir以增加白蚁体内miR-120的含量后，台湾乳白蚁的存活率降低约35%，绿僵菌对台湾乳白蚁的致死率提升约55%，同时显著抑制了台湾乳白蚁对绿僵菌的趋避反应。双荧光素酶报告系统等试验证实了miR-120靶向结合免疫基因Draper。通过对Draper进行注射法RNAi后，得到了类似于注射miR-120 agomir后的结果。研究结果表明，miR-120通过抑制靶基因Draper的表达，抑制了台湾乳白蚁抵御绿僵菌的免疫反应，最终导致台湾乳白蚁的存活率显著下降。该结果有助于进一步理解白蚁的免疫机制，并为开发抑制白蚁免疫防御反应的技术方法提供理论基础，有助于促进白蚁生物防治的发展。 |
| 英文摘要: |
| This study investigated the role of miR-120 in the immune response of Coptotermes formosanus against Metarhizium anisopliae through a series of experiments. First, injecting C. formosanus with miR-120 agomir to increase the level of miR-120 in C. formosanus decreased the survival rate of C. formosanus by about 35% and increased the mortality rate of C. formosanus treated with M. anisopliae to about 55%. At the same time, the repellent response of C. formosanus to M. anisopliae was significantly inhibited. Following, some experiments including the dual luciferase reporter system were performed to confirm that miR-120 targets the immune gene Draper. Finally, by injecting RNAi to knockdown of Draper and obtained results similar to those obtained after injection of miR-120 agomir. These results suggested that miR-120 inhibits the immune response of C. formosanus to M. anisopliae by inhibiting the expression of the target gene Draper, which ultimately leaded to a significant decrease in the survival rate of C. formosanus. This study will help us to better understand the immune mechanism of termites and provide a theoretical basis for the development of technical methods to inhibit termite immune responses, which will help to promote the development of biological control of termites. |
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