| 贺韶韶，余宙，石瑞琪，庄浩楠，陈功，于欢,2024,抗虫辣椒叶片对斜纹夜蛾幼虫相关生理生化指标的影响[J].环境昆虫学报,(3):650-658 |
| 抗虫辣椒叶片对斜纹夜蛾幼虫相关生理生化指标的影响 |
| Effect of insect resistant chili pepper leaves on physiological and biochemical indicators related to Spodoptera litura larvae |
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| DOI： |
| 中文关键词: 斜纹夜蛾 辣椒 生长发育 昆虫激素 酶活 |
| 英文关键词:Spodoptera litura Capsicum annuum growth and development insect hormones enzyme activity |
| 基金项目:国家自然科学基金（32172408）；湖南省现代农业产业技术体系项目（2022-31，2022-42） |
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| 中文摘要: |
| 为明确朝天椒(品种：BLTY2)叶片饲喂的斜纹夜蛾Spodoptera litura幼虫生长发育受到抑制的关键原因，本研究以人工饲料及牛角椒（品种：FXBX）叶片饲喂的斜纹夜蛾幼虫为对照，通过比较3种食料饲喂后斜纹夜蛾幼虫相关生理生化指标的变化，分析BLTY 2辣椒叶片饲喂所致的斜纹夜蛾幼虫生长发育异常的原因。结果表明，朝天椒辣椒叶片饲喂的斜纹夜蛾幼虫生长发育速率滞后于牛角椒辣椒叶片及人工饲料饲喂的斜纹夜蛾幼虫，且每头幼虫的发育时长均显著延长（P<0.05）。饲喂后第1天、第3天、第4天、第5天、第6天和第7天，饲喂BLTY 2辣椒叶片的斜纹夜蛾幼虫体内保幼激素（JH）含量均显著高于其他2种食料饲喂的斜纹夜蛾幼虫（P<0.05）。饲喂后第3天、第4天、第5天和第7天，饲喂BLTY 2辣椒叶片的斜纹夜蛾幼虫体内蜕皮激素（Ecd）含量均显著高于其他2种食料饲喂的斜纹夜蛾幼虫（P<0.05）。3种食料饲喂后斜纹夜蛾幼虫体内的几丁质酶（CHT）活性变化差异较大，而2种辣椒叶片饲喂后斜纹夜蛾幼虫体内的组织蛋白酶（CTS）活性变化趋势相似，且不同于人工饲料饲喂的斜纹夜蛾幼虫体内的CTS活性变化趋势。由此可见，BLTY 2辣椒主要干扰斜纹夜蛾幼虫体内JH与Ecd的含量抑制其生长发育。本研究结果可为筛选具有抗虫活性的植物次生代谢物质奠定基础。 |
| 英文摘要: |
| Identify the key reasons for the inhibition of the growth and development of Spodoptera litura larvae fed with the leaves of Chaotian pepper. Using artificial diet and leaves of cowhorn pepper as control, by comparing the changes in physiological and biochemical indicators of S. litura larvae fed with three different diets to analyze the reasons for abnormal growth and development of S. litura larvae fed with Chaotian pepper （Variety:BLTY 2） chill pepper leaves. The results showed that the growth and development rate of the larvae fed with BLTY 2 chill pepper leaves was slower than that of the larvae fed with Niujiao pepper(Variety:FXBX) chill pepper leaves and artificial diet, and the developmental time of each larval stage of FXBX chill pepper leaves fed larvae were significantly prolonged (P<0.05). On the 1st, 3rd, 4th, 5th, 6th, and 7th days after feeding, the juvenile hormone (JH) content in the body of larvae fed with BLTY 2 chill pepper leaves was significantly higher than that of larvae fed with the other two diets (P<0.05). On the 3rd, 4th, 5th, and 7th days after feeding, the ecdysone (Ecd) content in the body of larvae fed with BLTY 2 chill pepper leaves was significantly higher than that of larvae fed with the other two diets (P<0.05). The changes in chitinase (CHT) activity in the body of tested larvae fed with three different diets showed significant differences, while the changes of cathepsin (CTS) activity in the body of S. litura larvae fed with two types of chili pepper leaves was similar and different from that of artificial diet fed larvae. The results indicated that BLTY 2 chill pepper mainly inhibits the growth and development of S. litura larvae by interfering with the content of JH and Ecd in their bodies. The results obtained in this study lay the foundation for the screening of plant secondary metabolites with insect resistant activity. |
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