| 李德伟，罗亚伟，黄杏，易婷婷，覃振强,2023,高温胁迫对新菠萝灰粉蚧不同地理种群保护酶活性的影响[J].环境昆虫学报,(3):659-665 |
| 高温胁迫对新菠萝灰粉蚧不同地理种群保护酶活性的影响 |
| Effects of high temperature stress on the activities of three enzymes of Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcidae) in different geographical populations |
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| DOI： |
| 中文关键词: 新菠萝灰粉蚧 高温胁迫 过氧化物酶 酚氧化酶 谷胱甘肽-S-转移酶 |
| 英文关键词:Dysmicoccus neobrevipes high temperature stress peroxidase phenol oxidase glutathione-S-transferase |
| 基金项目:国家自然科学基金（31760540）；广西农业科学院基本科研业务专项（2021YT013） |
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| 中文摘要: |
| 为分析高温胁迫下新菠萝灰粉蚧Dysmicoccus neobrevipes Beardsley不同地理种群保护酶活性的差异，阐明该粉蚧对高温适应性的生理响应。本研究以室内26℃处理为对照测定了高温胁迫下（35℃、38℃、41℃、44℃）新菠萝灰粉蚧4个不同地理（广西、广东、海南和云南）种群雌成虫过氧化物酶（POD）、酚氧化酶（PO）及谷胱甘肽-S-转移酶（GST）的活性。结果表明，在35~44℃高温胁迫下，新菠萝灰粉蚧不同地理种群雌成虫POD和GST活性均高于常温对照的，PO活性均低于常温对照的（除云南种群38℃处理外）；在35~44℃高温胁迫下该粉蚧3种酶活性变化均具有随着处理温度的升高呈先升高后降低趋势。在常温26℃下，该粉蚧除了广西、广东种群POD活性显著高于海南、云南种群外，4个种群的PO、GST间的活性无显著差异。在38℃、41℃和44℃高温处理下广西、广东、云南种群间的POD活性无显著差异。在相同高温处理下，除广东种群38℃处理的GST活性显著低于其它种群的外，其它相同温度处理的不同种群间的GST活性无显著差异。说明新菠萝灰粉蚧广西、广东、云南种群的POD对38℃、41℃、44℃高温适应性和该粉蚧4个种群的GST对35℃、41℃、44℃高温适应性均无明显差异。而高温胁迫对不同地理种群粉蚧的酶活性影响不同，不同地理种群的POD、PO和GST活性经不同的高温胁迫后出现了显著差异。本研究表明高温对新菠萝灰粉蚧不同地理种群雌成虫的POD、PO和GST活性有重要影响，POD及GST对该粉蚧抵抗高温胁迫起到积极作用，而PO可能在应对高温胁迫中没起到积极的抵抗作用，而是通过调节体内POD、GST活性变化来适应高温的胁迫。 |
| 英文摘要: |
| The differences of enzyme activities of Dysmicoccus neobrevipes from different geographical populations under high temperature stress were analyzed and the physiological response of this mealybug to high temperature adaptation was elucidated in this study. The activities of peroxidase (POD), phenol oxidase (PO) and glutathione-S-transferase (GST) in female adults of D. neobrevipes from four different geographical populations (Guangxi, Guangdong, Hainan and Yunnan) under high temperatures stress (35℃, 38℃, 41℃ and 44℃) were determined in this paper, and the control temperature was at 26℃. The results showed that the activities of POD and GST in female adult of D. neobrevipes from different geographical populations were higher than those of at 26℃, and the activities of PO were lower than those of at 26℃ (except Yunnan population at 38℃) under high temperature stress of 35~44℃. The activities of three enzymes increased first and then decreased with the increase of temperature under high temperature stress of 35~44℃. At 26℃, except the POD activities of Guangxi and Guangdong populations were significantly higher than those of Hainan and Yunnan populations, there were no significant differences in the activities of PO and GST among the four populations. There were no significant differences in POD activities among the populations in Guangxi, Guangdong and Yunnan under 38℃, 41℃ and 44℃ high temperature treatment. Under the same high temperature, except the GST activity of Guangdong population at 38℃ was significantly lower than that of other populations, there were no significant differences in GST activities among different populations at the same temperature. This study indicated that there were no significant differences in the adaptabilities of POD to 38℃, 41℃, 44℃ in the populations of Guangxi, Guangdong, Yunnan and GST to 35℃, 41℃, 44℃ in all the populations. The effects of high temperature stress on enzyme activities of different geographical populations were different. The activities of POD, PO and GST of different geographical populations were significantly differences under different high temperature stress. Therefore, high temperature had an impact on the activities of POD, PO and GST in different geographical populations of D. neobrevipes female adults. POD and GST play a positive role in resisting high temperature stress, while PO may not play an active role in the resistance to the mealybug to high temperature stress. The female adults of D. neobrevipes was able to adapt to high temperature stress by adjusting the activities of POD and GST. |
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