| 齐琪，孙丽丽，许力山，曹传旺,2021,RNAi分析舞毒蛾谷胱甘肽S-转移酶（GST）基因对黄酮和槲皮素胁迫响应[J].环境昆虫学报,43(6):1359-1367 |
| RNAi分析舞毒蛾谷胱甘肽S-转移酶（GST）基因对黄酮和槲皮素胁迫响应 |
| Response of glutathione S-transferase (GST) genes in Lymantria dispar to flavone and quercetin stresses based on RNAi analysis |
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| DOI： |
| 中文关键词: 舞毒蛾 RNAi 谷胱甘肽S-转移酶 黄酮 槲皮素 |
| 英文关键词:Gypsy moth RNAi glutathione S-transferase (GST) flavone quercetin |
| 基金项目:国家重点研发计划项目（2018YFC1200400） |
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| 中文摘要: |
| 为了明确舞毒蛾Lymantria dispar谷胱甘肽S-转移酶家族基因对生长发育影响及对次生物质的响应机制，采用RNAi技术分别沉默LdGSTe2、LdGSTs1、LdGSTs2和LdGSTz1基因，分析对舞毒蛾3龄幼虫体重、存活率、营养利用等生理指标的影响以及次生物质黄酮和槲皮素胁迫响应。结果表明，dsRNA可有效抑制LdGSTe2、LdGSTs1、LdGSTs2和LdGSTz1基因表达，分别注射dsLdGSTe2、dsLdGSTs1、dsLdGSTs2和dsLdGSTz1处理组舞毒蛾幼虫的相对生长率、相对取食量、食物利用率、食物转化率均低于对照组，但对幼虫的存活率无影响，表明LdGSTe2、LdGSTs1、LdGSTs2和LdGSTz1影响舞毒蛾幼虫生长发育；次生物质黄酮和槲皮素胁迫下，沉默LdGSTe2、LdGSTs1、LdGSTs2和LdGSTz1后舞毒蛾幼虫存活率显著下降，体重显著降低。沉默LdGSTe2、LdGSTs1、LdGSTs2和LdGSTz1基因对舞毒蛾幼虫的生长发育、食物利用具有抑制作用，同时影响了舞毒蛾幼虫对黄酮和槲皮素的适应能力。 |
| 英文摘要: |
| To clarify the effects of glutathione S-transferase (GST) genes on growth and development of Lymantria dispar and its response to secondary metabolite stresses, RNAi silencing technology was used to analyze the specific function of LdGSTe2, LdGSTs1, LdGSTs2 and LdGSTz1 genes. The effects of GST genes on body weight, survival rate, nutritional utilization and the stress responses of flavone and quercetin were analyzed in 3rd instar L. dispar larvae. The results showed that dsRNA could effectively inhibit the expression of LdGSTe2, LdGSTs1, LdGSTs2 and LdGSTz1 genes. Analysis of nutritional utilization showed that the relative growth ratio, the relative consumption rate, the efficiency of conversion of ingested food and the efficiency of conversion of digested food of L. dispar larvae microinjected by dsGFP was higher than those microinjected by dsGSTs, but had no effect on the survival rate. The results showed that LdGSTe2, LdGSTs1, LdGSTs2 and LdGSTz1 affected the growth and development of L. dispar larvae. However, under the stress of flavone and quercetin, the mortality of dsRNA treated L. dispar larvae increased, and the weight cumulative growth rates of L. dispar larvae were decreasing. Silencing of LdGSTe2, LdGSTs1, LdGSTs2 and LdGSTz1 genes inhibited the growth, development and food utilization of L. dispar larvae, and affected the adaptability ability of L. dispar larvae to flavonoid and quercetin. |
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