| 蔡腾，李嘉希，李志强，毛洪，李喜贵，单体江,2021,黑水虻幼虫氨基酸及其微量元素分析[J].环境昆虫学报,(3):684-689 |
| 黑水虻幼虫氨基酸及其微量元素分析 |
| Analysis of amino acid and trace element of Hermetia illucens larvae |
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| DOI： |
| 中文关键词: 黑水虻 幼虫 氨基酸 微量元素 |
| 英文关键词:Hermetia illucens Larvae Amino acid Element analysis |
| 基金项目:广东省现代农业产业共性关键技术研发创新团队建设项目（2019KJ134） |
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| 中文摘要: |
| 黑水虻具有较高的营养价值，是一种重要的环境友好型资源昆虫。本研究采用凯氏定氮法、茚三酮柱后衍生离子交换色谱法和微波消解-电感耦合等离子质谱法（ICP-MS）等多种分析技术，测定了厨余垃圾饲喂的黑水虻幼虫蛋白质、氨基酸和微量元素的组成及含量。研究结果表明，黑水虻幼虫中蛋白质的含量为40.60 g/100 g。在测定的16种氨基酸中含量最高的是谷氨酸（GLU），含量为4.88 g/100 g，其次是天冬氨酸（ASP），含量为3.31 g/100 g；有7种氨基酸的含量超过2.00 g/100 g，16种水解氨基酸的总和为36.16 g/100 g，必需氨基酸占总氨基酸含量的46.35%。从黑水虻幼虫中共检测出28种微量元素，其中钙元素的含量最高，为25 787 mg/kg，其次是K、Na、Mg和Fe，含量分别为7 684 mg/kg、1 358 mg/kg、964 mg/kg和317 mg/kg；Al、Zn、Sr和Mn的含量在20～100 mg/kg之间，其他微量元素的含量均在10 mg/kg以下。黑水虻幼虫中有害元素含量均低于限量，符合我国现行的饲料卫生标准GB 13078-2017。本研究为黑水虻幼虫资源的开发和利用提供了重要的理论依据。 |
| 英文摘要: |
| Hermetia illucens are important environmental friendly and resource-oriented insects with high nutritional value. The compositions and contents of proteins, amino acids and trace elements in H.illucens larvae fed with kitchen wastes were determined by Kjeldahl method, ion exchange chromatography with ninhydrin post-column derivatization and microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS)method, respectively. The results showed that the protein content of H.illucens larvae was 40.60 g/100 g. Glutamate (GLU)had the highest content of 4.88 g/100 g, followed by aspartic acid (ASP)which was 3.31 g/100 g among the sixteen determination amino acids; the contents of seven amino acids were more than 2.00 g/100 g, and the total content of 16 hydrolyzed amino acids was 36.16 g/100 g. The essential amino acids accounted for 46.35 % of the total amino acids. There were a total of 28 trace elements, and the content of calcium with 25 787 mg/kg was the highest, followed by K, Na, Mg and Fe, which were 7 684 mg/kg, 1 358 mg/kg, 964 mg/kg and 317 mg/kg, respectively; the contents of Al, Zn, Sr and Mn varied from 20 mg/kg to 100 mg/kg, and other trace elements were all below 10 mg/kg. The contents of harmful elements in Hermetia illucens larvae were all lower than the limit, which met the National Feed Hygiene Standard (GB 13078-2017). This study provided an important theoretical basis for the development and utilization of the larva resources of H.illucens. |
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