| 柳 青,李 燕，王德海，罗 嵘，土小佳, 黄钰淼，傅 杨* ,陈国华*,2016,基于ISSR分子标记技术的稻水象甲遗传多样性研究[J].环境昆虫学报,(1):102-112 |
| 基于ISSR分子标记技术的稻水象甲遗传多样性研究 |
| Research on genetic diversity of the rice water weevil, Lissorhoptrus oryzophilus Kuschel （Coleoptera: Curculionidae）ased on ISSR markers |
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| DOI： |
| 中文关键词: 稻水象甲 ISSR 遗传多样性 遗传分化 |
| 英文关键词:Lissorhoptrus oryzophilus Kuschel ISSR genetic diversity genetic differentiation |
| 基金项目:农业部农函(2007)3号 |
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| 中文摘要: |
| 稻水象甲Lissorhoptrus oryzophilus Kuschel是一种世界性的水稻害虫，已侵入我国23个省市区，发生面积近千万亩。为弄清我国不同地区稻水象甲的遗传多样性水平、居群间的遗传分化及可能的扩散路线，本研究采用ISSR分子标记技术分析了我国13个省28个地区稻水象甲样本的遗传多样性。利用15条ISSR引物共扩增得到258条带，其中247条为多态性条带。在物种水平上，稻水象甲的多态位点百分率（PPB）为95.73%，Nei's基因多样性（H）为0.3578，Shannon信息多样性指数 （I） 为0.5330；在居群水平上，以上3项指标的平均值分别为46.28%、0.768、0.2620。Nei's基因分化系数 Gst 为0.5059，表明有50.59%的遗传变异存在于居群间，49.41%的遗传变异存在于居群内，居群间表现出较高水平的遗传分化。通过UPGMA聚类可将稻水象甲28个居群分成2大支系：安徽池州、安徽合肥、云南嵩明、云南曲靖、江西南昌、四川盐亭、辽宁盘锦、辽宁东港、山西太原、山东东营、天津蓟县、陕西城固、河北唐山、云南寻甸等14个居群聚成第一个分支；贵州、贵州开阳、贵州龙里、贵州贵阳、贵州修文、四川隆昌、四川泸州、湖北武当山、湖北荆门、湖北利川、湖南宁乡、湖南双峰、湖南邵阳、天津宝坻等聚成第二个分支 |
| 英文摘要: |
| The rice water weevil, Lissorhoptrus oryzophilus Kuschel, is one of the most destructive insect pests of rice, having invaded into 23 Provinces and nearly 10 million acres of paddies have been infested by the pest in mainland China. In order to verify the genetic diversity, genetic variation and the possible spread routes of this pest, the genetic diversity of L.oryzophilus collected from 28 areas of 13 provinces was evaluated using the inter-simple sequence repeat (ISSR) molecular markers. The results showed that fifteen ISSR primers produced a total of 258 bands, of which 247were polymorphic. At species level, the percentage of polymorphic bands (PPB) was 95.73%, Nei’s information index (H) was 0.3578, Shannon’s Information index (I)was 0.5330; at population level, the average value of the three indexes were 46.28%, 0.1768 and 0.2620, respectively. The coefficient of genetic differentiation among populations Gst was 0.5059, indicating most of genetic variation partitioned among populations (50.59%), 49.41% within populations. Two distinct clades were recognized from the dendrogram of 28 populations of L. oryzophilus: Anhui Chizhou (AHCZ), Anhui Hefei (AHHF), Yunnan Songming (YNSM), Yunnan Qujing (YNQJ), Jiangxi Nanchang (JXNC), Sichuan Yanting (SCYT), Liaoning Panjin (LNPJ), Liaoning Donggang (LNDG), Shanxi Taiyuan (SXTY), Shandong Dongying (SDDY), Tianjin Jixian (TJJX), Shanxi Chenggu (SXCG), Hebei Tangshan (HBTS) and Yunnan Xundian (YNXD) formed as the first clade, while the other populations, Guizhou (GZ), Guizhou Kaiyang (GZKY), Guizhou Longli (GZLL), Guizhou Guiyang (GZZZ), Guizhou Xiuwen (GZXW), Sichuan Longchang (SCLC), Sichuan Luzhou (SCLZ), Hubei Wudang mountain (HBWD), Hubei Jingmen (HBJM), Hubei Lichuan (HBLC), Hunan Ningxiang (HNNX), Hunan Shuangfeng (HNSF), Hunan Shaoyang (HNSY) and Tianjin Baodi (TJBD), formed as the second clade. It was speculated that the dispersal routes of L.oryzophilus were complicated and multidirectional, which showed a common characteristic, that the rice water weevil invaded initially into coastal areas through the port trade and transportation, and then spread across other inland provinces along the railway or highway transportation. Of course, more research was needed with DNA sequences methods and the knowledge of phylogeography to investigate the diffusion process and genetic differentiation time of this pest. |
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