《环境昆虫学报》

将不同浓度Ni2+添入人工饲料中，研究连续3代取食含不同剂量Ni2+饲料的斜纹夜蛾5、6龄幼虫血细胞对凝胶珠包囊反应的影响。将新蜕皮的5龄幼虫记为0 h，每隔24 h注射凝胶珠处理幼虫，直至6龄末，注射24h后解剖虫体，观察包囊情况。结果表明，5mg/kg及以下处理浓度Ni2+的1代胁迫可提高0-120 h幼虫血细胞对凝胶珠的包囊反应，但2-3代的胁迫只增加发育初期0-72 h幼虫血细胞的包囊反应；10-20 mg/kg处理浓度Ni2+的1代胁迫仅增加0-24 h幼虫血细胞的包囊率，但可降低2-3代幼虫血细胞各发育时间的包囊率。此外，40 mg/kg Ni2+胁迫可连续降低3个世代各个发育阶段幼虫的包囊反应。饲料中Ni2+剂量、胁迫世代数以及两者的交互作用均对幼虫包囊率产生了显著影响。

英文摘要:

Encapsulation rate of hemocytes of the 5th and 6th instar larvae of Spodoptera litura Fabricius were investigated at 24 h interviews after the larvae were fed on the artificial diets with different doses of nickel for 3 generations. The time of newly moulted 5thinstar larvae was labeled as 0 h and the end time of the 6th instar larvae was recorded as 120 h. Results showed It was indicated that one generation’s nickel exposure with lower levels of nickel (≤5mg/kg) improved the encapsulation response of larvae at the time of 0-120 h, while two and three generations’ nickel exposure only increased the encapsulation response of larvae at the time of 0-72 h. Encapsulation rate of larvae exposed to higher level of nickel (10-20 mg/kg) was only improved after one generation’s nickel exposure in larvae at 0-24 h. However, long term exposure with higher levels of nickel inhibited immune response of larvae at the all developmental stage. Moreover, 40 mg/kg nickel exposure reduced Encapsulation rate of both 5th and 6th instar larvae from all treatments. Results also indicated that nickel doses in diet, exposing generation and nickel doses in diet × exposing generation all have significant effect on the encapsulation of the treated time of the 5th and 6th instar larvae.

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