| 李玉辉,李新浩,刘盼,林莉,杨思华,武目涛,吴晓薇,胡淑青,李盼畔,马骏,刘海军,2026,基于线粒体COI DNA条形码序列的菜豆象种群遗传分化与种群结构分析[J].环境昆虫学报,(4):1056-1066 |
| 基于线粒体COI DNA条形码序列的菜豆象种群遗传分化与种群结构分析 |
| Population genetic differentiation and structure analysis of the bean Weevil, Acanthoscelides obtectus (Say) based on mitochondrial COI DNA barcode sequences |
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| DOI：10.3969/j.issn.1674-0858.2026.04.8 |
| 中文关键词: 菜豆象 种群遗传结构 COI 单倍型 |
| 英文关键词:Acanthoscelides obtectus (Say) population structure COI haplotype |
| 基金项目:国家重点研发计划（2021YFD1400104） |
| 作者 | 单位 | | 李玉辉 | 1. 广州海关技术中心,广州 510623 | | 李新浩 | 2. 广州白云机场海关综合技术服务中心,广州 510440 | | 刘盼 | 3. 华南农业大学,广州 510640 | | 林莉 | 1. 广州海关技术中心,广州 510623 | | 杨思华 | 1. 广州海关技术中心,广州 510623 | | 武目涛 | 1. 广州海关技术中心,广州 510623 | | 吴晓薇 | 1. 广州海关技术中心,广州 510623 | | 胡淑青 | 1. 广州海关技术中心,广州 510623 | | 李盼畔 | 1. 广州海关技术中心,广州 510623 | | 马骏 | 1. 广州海关技术中心,广州 510623 | | 刘海军 | 1. 广州海关技术中心,广州 510623 |
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| 中文摘要: |
| 菜豆象Acanthoscelides obtectus（Say）是全球性的重要仓储害虫，每年因豆类粮食被侵蚀而造成严重经济损失。【目的】为了揭示国内外菜豆象种群遗传分化与种群结构。【方法】本研究基于线粒体COI条形码序列，对亚洲、欧洲、非洲和南美洲的21个地理种群开展种群地理分布、种群遗传多样性和种群遗传分化研究。【结果】种群地理分布分析发现菜豆象种群分布广泛，遍及亚洲、欧洲、非洲和南美洲。单倍型分析发现菜豆象共有15种单倍型（H1~H15），而且单倍型分布不均衡，显示出地理偏好性。遗传多样性分析发现菜豆象种群具有高单倍型多样性，但是核苷酸多样性低（单倍型多样性指数Hd=0.665、核苷酸多样性指数Pi=0.00291），并且具有种群间差异。单倍型网络和进化分析发现菜豆象种群结构呈现多中心点辐射模式，而且种群间出现一定程度的遗传分化，分成3个亚群。种群遗传分化和基因流分析表明菜豆象种群间出现一定程度的遗传分化，基因交流程度具有较大差异（欧洲-非洲之间Fst=0.0851、Nm=2.6881；南美洲-亚洲之间Fst=0.7690、Nm=0.0751），而且遗传分化与地理距离基本无相关性（r=0.2102，P=0.031< 0.05）。中性检验表明乌干达（UGA）种群（Tajima's D=-1.79107，P < 0.05）可能在近期出现了种群扩张，而其余20个菜豆象地理种群符合中性突变，群体大小相对稳定。【结论】本研究为进一步阐明国内外菜豆象扩散来源和入侵路径，进而构建可持续防控体系奠定基础。 |
| 英文摘要: |
| The bean weevil, Acanthoscelides obtectus (Say), is a globally important storage pest which caused severe economic losses every year due to the erosion of legumes.【Aim】In order to reveal the genetic differentiation and population structure of A. obtectus.【Methods】We investigated the population geographical distribution, population genetic diversity and population genetic differentiation of 21 geographic populations from Asia, Europe, Africa, and South America based on mitochondrial COI DNA barcode sequences.【Results】Geographical distribution analysis revealed widespread dispersal of A. obtectus populations across all four continents. Haplotype analysis showed there were 15 distinct haplotypes (H1~H15), and they were unevenly distributed, showing geographical preference. Genetic diversity analysis showed that A. obtectus populations had high haplotype diversity but low nucleotide diversity (Hd=0.665, Pi=0.00291), and there were differences among populations. Haplotype network and phylogenetic analysis showed that the population structure of A. obtectus was a multi-hub-and-spoke radiation pattern, and there was a certain degree of genetic differentiation among the populations, which were divided into three subgroups. Genetic differentiation and gene flow analysis revealed that there was a certain degree of genetic differentiation among the populations, and the degree of gene flow was greatly different (e.g., Fst=0.0851, Nm=2.6881 between Europe and Africa; Fst=0.7690, Nm=0.0751 between South America and Asia). There was no significant correlation between genetic differentiation and geographic distance (r=0.2102, P=0.031<0.05). Neutral tests suggested recent population expansion in the Uganda population (Tajima's D=-1.79107, P<0.05), while the remaining 20 populations conformed to neutral mutation expectations with stable effective population sizes.【Conclusion】This study laid a foundation for further understanding the spread sources and invasion routes of A. obtectus at home and abroad, and building a sustainable prevention and control system. |
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