| 何祝清,徐菁忆,陈龙,2026,DNA条形码在中国棺头蟋属鉴定中的应用[J].环境昆虫学报,(4):1017-1024 |
| DNA条形码在中国棺头蟋属鉴定中的应用 |
| Application of DNA barcoding in identification of the genus Loxoblemmus in China |
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| DOI：10.3969/j.issn.1674-0858.2026.04.4 |
| 中文关键词: 棺头蟋属 COI基因 物种界定 DNA条形码 |
| 英文关键词:Loxoblemmus COI gene species delimitation DNA barcoding |
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| 中文摘要: |
| 【目的】棺头蟋属Loxoblemmus Saussure，1877全世界共记录有61种，我国记录18种。以往的分类学研究主要根据面部的形态特征进行区分，但也有研究指出同种的个体间该特征存在较大差异。【方法】本研究首先对27个棺头蟋样品进行形态学鉴定，再通过对样品的COI基因测序并结合15条NCBI数据库序列进行分子物种界定和构建系统发育树，对中国棺头蟋属物种鉴定进行了初步探究。【结果】通过COI基因可将27个样品划分为7~8个种，少于或有别于形态学鉴定的9个种。其中泰康棺头蟋L. taicoun的样本中可能存在隐存种或因地理隔离可能正处于物种分化过程。附突棺头蟋L. appendicularis和介棺头蟋L. intermedius存在雄性成虫头部多型现象，导致据此的形态学分类结果出现二者间的交错。多伊棺头蟋L. doenitzi的雄性头部形态多型现象，使其中部分个体被鉴定为窃棺头蟋L. detectus或哈尼棺头蟋L. haani，应将窃棺头蟋和哈尼棺头蟋在中国的棺头蟋名录中剔除。【结论】本研究揭示了传统形态学分类在棺头蟋属中的局限性，验证了DNA条形码在该类群鉴定中的可行性，为后续分类修订提供了可行方案。 |
| 英文摘要: |
| 【Aim】The genus Loxoblemmus Saussure, 1877 comprises 61 described species worldwide, with 18 species recorded in China. Previous taxonomic studies have primarily relied on facial morphological characters for species identification, despite notable intraspecific variation reported in these traits.【Methods】In this study, we integrated morphological examination (27 samples) with molecular analysis (COI gene sequences from the same 27 samples and additional sequences from NCBI) to conduct a preliminary reassessment of species identification in Chinese Loxoblemmus crickets.【Results】Molecular delimitation based on COI gene sequences grouped the 27 samples into 7~8 putative species, demonstrating incongruence with the 9 morphospecies identified. Cryptic species might exist within the samples of L. taicoun, or they may be undergoing speciation due to geographic isolation. Moreover, males of L. appendicularis and L. intermedius exhibit pronounced head polymorphism, leading to overlapping and ambiguous morphological identifications between these two species. The high degree of male head polymorphism in L. doenitzi had resulted in some individuals being identified as L. detectus or L. haani. It is proposed that L. detectus and L. haani should be removed from the Chinese Loxoblemmus checklist.【Conclusion】In summary, this study reveals the limitations of traditional morphological taxonomy in the genus Loxoblemmus, confirms the feasibility of DNA barcoding for species identification in this genus, and proposes practical solutions for future taxonomic revisions. |
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