| 任金梁,马茁,张润志,2026,中国象虫总科（Coleoptera：Curculionoidea）DNA条形码研究[J].环境昆虫学报,(4):1009-1016 |
| 中国象虫总科（Coleoptera：Curculionoidea）DNA条形码研究 |
| DNA barcoding of Chinese weevils (Coleoptera: Curculionoidea) |
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| DOI：10.3969/j.issn.1674-0858.2026.04.3 |
| 中文关键词: DNA条形码 中国 象虫总科 COI-5′ 数据集 |
| 英文关键词:DNA barcoding China Curculionoidea COI-5′ dataset |
| 基金项目:国家自然科学基金（32470456） |
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| 摘要点击次数: 1410 |
| 全文下载次数: 85 |
| 中文摘要: |
| 【目的】DNA条形码是一段可用于物种快速、准确鉴定的标准化短DNA片段。然而，中国象虫总科的DNA条形码研究基础薄弱，不仅数据积累有限，更缺乏系统性的参考数据库，这在一定程度上制约了物种鉴定等研究的深入开展。本研究旨在为我国象虫类群的DNA条形码研究提供数据基础。【方法】通过整合公共数据库中的现有序列（409条）和自行测定的数据（1 459条），本研究初步构建了中国象虫总科的DNA条形码参考数据集。【结果】该数据集共涵盖571个物种（约占中国已知物种数的1/7）和1 868条COI序列，覆盖象虫总科中的5个科。其中，象虫科（483个物种，1 691条序列）在数据集中占主体地位，与其分类学上的优势地位相符。地理分布方面，数据来源于全国15个省级行政区，其中西藏、云南和四川三地的物种数占总数的80%以上，反映出青藏高原及周边区域较高的采样强度与潜在的丰富生物多样性。序列鉴定方面，51.8%的序列可被鉴定至种或属级水平。其中，自行测序的数据中鉴定到种级的比例（24.6%）显著高于公共数据库来源的数据（10.5%）。【结论】本研究初步构建了中国象虫总科首个覆盖多科、多地域的DNA条形码参考数据集，填补了该类群在参考数据库方面的空白，为后续物种鉴定、生物多样性监测及系统发育研究提供了重要的数据支撑。 |
| 英文摘要: |
| 【Aim】DNA barcoding relies on a short, standardized DNA fragment for rapid and accurate species identification. However, DNA barcoding research on Chinese weevils remains relatively underdeveloped. The lack of a systematic and comprehensive barcode database, in particular, has limited in-depth taxonomic studies in this group. This study aims to establish a foundational data resource for DNA barcoding of Chinese weevils.【Methods】By integrating publicly available sequences (409 sequences) with newly generated DNA barcode data (1 459 sequences), we preliminarily constructed a DNA barcode reference dataset for Chinese Curculionoidea.【Results】The dataset included 1 868 COI sequences representing 571 species-approximately one-seventh of the known species diversity in China-covering five families within the Curculionoidea. As expected, the family Curculionidae constituted the majority of the dataset, with 483 species and 1 691 sequences, reflecting its taxonomic dominance. Geographically, the data were sourced from 15 provincial-level regions across China, with over 80% of the species collected from Xizang, Yunnan, and Sichuan. This distribution highlighted both the intensive sampling efforts and the potentially high biodiversity of the Qinghai-Xizang Plateau region. In terms of sequence identification, our data yielded successful species- or genus-level identification for 51.8% of the sequences. Notably, the proportion of sequences identified to species level was significantly higher for our newly generated data (24.6%) than for public?database sequences (10.5%).【Conclusion】This study provides the first multi?family, multi?region DNA barcode reference dataset for Chinese Curculionoidea, filling a critical gap in the taxonomic reference infrastructure. The dataset offers a valuable resource for future species identification, biodiversity monitoring, and phylogenetic research on this ecologically and economically important group. |
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