| 冶晓莉,林伟强,李诗颖,杜澄举,王偲,吕春贺,李亭潞,庞锐,文超,孙思,,三种除草剂对红火蚁存活与行为的影响及分子机制研究[J].环境昆虫学报,(): |
| 三种除草剂对红火蚁存活与行为的影响及分子机制研究 |
| Effects of three herbicides on the survival and behavior of red imported fire ants and the underlying molecular mechanisms |
| |
| DOI： |
| 中文关键词: 红火蚁 除草剂 非靶标作用 挖掘行为 工具使用 致死中剂量 |
| 英文关键词:Solenopsis invicta herbicide non-target effect digging behavior tool use median lethal dose |
| 基金项目:国家自然科学基金（32301292）；北京市高创计划青年托举工程项目（20250671） |
| 作者 | 单位 | | 冶晓莉 | 1. 华南农业大学林学与风景园林学院,广州 510642 | | 林伟强 | 1. 华南农业大学林学与风景园林学院,广州 510642 | | 李诗颖 | 1. 华南农业大学林学与风景园林学院,广州 510642 | | 杜澄举 | 1. 华南农业大学林学与风景园林学院,广州 510642 | | 王偲 | 1. 华南农业大学林学与风景园林学院,广州 510642 | | 吕春贺 | 2. 广东省森林资源保育中心,广州 510173 | | 李亭潞 | 2. 广东省森林资源保育中心,广州 510173 | | 庞锐 | 3. 华南农业大学植物保护学院,广州 510642 | | 文超 | 4. 北京林业大学草业与草原学院,北京 100083 | | 孙思 | 1. 华南农业大学林学与风景园林学院,广州 510642 |
|
| 摘要点击次数: 55 |
| 全文下载次数: 5 |
| 中文摘要: |
| 【目的】近年来，红火蚁Solenopsis invicta已广泛入侵我国农林与城市生境，对农业生产、生态安全及居民健康造成了严重威胁。前期在广东省苗圃开展红火蚁调查时，从当地林农处获悉，部分除草剂可能兼具杀灭和驱避红火蚁的作用，从而实现除草与控蚁的双重效果。【方法】本研究选取3种生产上常用的除草剂（三氯吡氧乙酸丁氧基乙酯、氨氯吡啶酸和氟磺胺草醚），评估其对红火蚁存活及行为的影响，并利用转录组技术初步探究其分子机制。【结果】三氯吡氧乙酸丁氧基乙酯对红火蚁工蚁表现出较高的毒力，24 h、48 h和72 h的致死中剂量（LD50）分别为2.407 μg/头、1.509 μg/头和1.411 μg/头。此外，三氯吡氧乙酸丁氧基乙酯在5 mg/kg、50 mg/kg和500 mg/kg浓度下显著抑制红火蚁工蚁的挖掘行为，但对觅食行为的影响相对较弱；这可能是由于觅食红火蚁工蚁搬运大量土壤颗粒覆盖该药剂处理的表面，从而减少了与药剂的直接接触。相比之下，在本研究测试的浓度下（5 mg/kg、50 mg/kg和500 mg/kg），氨氯吡啶酸和氟磺胺草醚对红火蚁工蚁的存活与行为均无显著影响。转录组分析显示，三氯吡氧乙酸丁氧基乙酯处理后红火蚁工蚁的部分CYP450基因显著上调，而嗅觉相关基因和基础细胞功能通路显著下调。【结论】三氯吡氧乙酸丁氧基乙酯对红火蚁工蚁具有明显的毒性与驱避作用，而氨氯吡啶酸和氟磺胺草醚可能对蚁类的非靶标效应较小。本研究表明三氯吡氧乙酸丁氧基乙酯在农林生境的施用可能同时实现“除草”和“控蚁”，从而降低管理成本。 |
| 英文摘要: |
| 【Aim】In recent years, the red imported fire ant, Solenopsis invicta, extensively invaded agricultural, forest, and urban habitats in China and posed serious threats to agricultural production, ecological security, and public health. During preliminary surveys of fire ants in nurseries in Guangdong Province, we learned from local forest farmers that certain herbicides might also exhibit insecticidal and repellent activities against S. invicta, thereby potentially providing both weed control and fire ant suppression.【Methods】In this study, three commonly used herbicides (triclopyr-2-butoxyethyl ester, picloram, and fomesafen) were selected to evaluate their effects on the survival and behavior of S. invicta, and transcriptomic analysis was employed to preliminarily explore the underlying molecular mechanisms.【Results】Triclopyr-2-butoxyethyl ester exhibited high toxicity to S. invicta workers, with median lethal dose (LD50) values of 2.407 μg/ant, 1.509 μg/ant and 1.411 μg/ant at 24 h, 48 h and 72 h, respectively. Additionally, at concentrations of 5 mg/kg, 50 mg/kg and 500 mg/kg, triclopyr-2-butoxyethyl ester significantly inhibited the digging behavior of S. invicta workers but had a relatively weak effect on foraging behavior. This was likely because foraging S. invicta workers transported large amounts of soil particles onto the herbicide-treated surface, thereby reducing their direct contact with the herbicide. In contrast, at the tested concentrations (5 mg/kg, 50 mg/kg and 500 mg/kg), picloram and fomesafen had no significant effects on the survival or behavior of S. invicta workers. Transcriptomic analysis revealed significant upregulation of some CYP450 genes and significant downregulation of olfactory-related genes and basal cellular function pathways in S. invicta workers following treatment with triclopyr-2-butoxyethyl ester.【Conclusion】Triclopyr-2-butoxyethyl ester exhibited marked toxicity and repellent activity against S. invicta workers, whereas picloram and fomesafen were likely to have relatively low non-target effects on ants. This study suggested that the application of triclopyr-2-butoxyethyl ester in agricultural and forestry habitats could simultaneously achieve weed control and fire ant management, thereby reducing management costs. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
