| 温乙妮，丁嘉欣，顾 欣，王新谱,2024,低温胁迫对番茄潜叶蛾体内抗寒物质含量的影响[J].环境昆虫学报,(6):1367-1373 |
| 低温胁迫对番茄潜叶蛾体内抗寒物质含量的影响 |
| Effect of low-temperature stress on the content of cold-resistant substances in Tuta absoluta |
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| DOI： |
| 中文关键词: 番茄潜叶蛾 低温胁迫 耐寒性 抗寒物质 越冬虫态 |
| 英文关键词:Tuta absoluta low temperature tolerance cold tolerance physiological and biochemical substances overwintering insect form |
| 基金项目:宁夏重点研发计划重点项目（2023BCF01045） |
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| 摘要点击次数: 13 |
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| 中文摘要: |
| 为明确番茄潜叶蛾Tuta absoluta在低温胁迫下幼虫、蛹和成虫体内抗寒物质含量的变化，本研究通过测定并分析4℃低温胁迫下各虫态体内水分、脂肪含量和抗寒物质（蛋白质、海藻糖和山梨醇）的含量变化，以期明确番茄潜叶蛾不同虫态的抗寒物质及其特征。结果表明，低温显著降低了幼虫和蛹的含水量，分别由74.46% ± 1.64%下降至68.66% ± 0.90%、70.23% ± 0.65%下降至66.92% ± 0.93%；低温使幼虫脂肪含量显著减少，由227.64 ± 6.28 μmol/mL显著降低至186.29 ± 6.28 μmol/mL。然而低温下各虫态蛋白质含量均显著增加，以蛹期增幅最大，由405.03 ± 13.678 μg/mg显著上升至554.82 ± 29.41 μg/mg，增加量达到149.79 ± 29.41 μmol/mg；蛹期体内海藻糖和山梨醇显著积累，分别由31.44 ± 0.48 mg/mL显著上升至39.82 ± 1.07 mg/mL，49.41 ± 0.72 mg/mL显著上升至52.15 ± 0.68 mg/mL，表明是蛹期的关键抗寒物质。因此，番茄潜叶蛾不同虫态对低温均有响应，其中蛹可能依赖蛋白质、海藻糖和山梨醇在宁夏地区露地越冬。 |
| 英文摘要: |
| In order to clarify the changes of cold-resistant substances in larvae, pupae and adults of the Tuta absoluta under low-temperature stress, the changes in the contents of water, fat content and cold-resistant substances (proteins, alginate and sorbitol) in each insect state were measured and analysed under low-temperature stress at 4℃, with a view to clarifying the cold-resistant substances and their characteristics in different insect states of the T. absoluta. The results showed that low temperature significantly reduced the water content of larvae and pupae from 74.46% ± 1.64% to 68.66% ± 0.90% and 70.23% ± 0.65% to 66.92% ± 0.93%, respectively; the fat content of larvae was significantly reduced from 227.64 ± 6.28 μmol/mL to 186.29 ± 6.28 μmol/mL under low temperature; while the fat content of each insect state was significantly reduced from 227.64 ± 6.28 μmol/mL to 186.29 ± 6.28 μmol/mL under low temperature. The protein content of all insect forms increased significantly at low temperature, with the greatest increase in the pupal stage, from 405.03 ± 13.678 μg/mg to 554.82 ± 29.41 μg/mg, an increase of 149.79 ± 29.41 μmol/mg. Alginate and sorbitol accumulated significantly in the pupal stage, from 31.44 ± 0.48 mg/mL to 39.82 ± 1.8 mg/mL, respectively. The significant accumulation of alginate and sorbitol from 31.44 ± 0.48 mg/mL to 39.82 ± 1.07 mg/mL and from 49.41 ± 0.72 mg/mL to 52.15 ± 0.68 mg/mL,respectively during the pupal stage indicated that they were the key chilling resistance substances during the pupal stage. Therefore, different insect forms of T.absoluta responded to low temperatures, among which the pupae of T.absoluta may rely on proteins, alginate and sorbitol to overwinter in the open ground in Ningxia. |
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